高等学校化学学报 ›› 2011, Vol. 32 ›› Issue (6): 1272.

• 研究论文 • 上一篇    下一篇

环氧树脂微流控芯片的表面功能化修饰

王琳琳1,2, 黄江1, 金伟1, 金钦汉1, 牟颖1,2   

  1. 1. 浙江大学工业控制技术国家重点实验室, 智能系统与控制研究所, 分析仪器研究中心, 杭州 310058;
    2. 吉林大学分子酶学工程教育部重点实验室, 长春 130021
  • 收稿日期:2010-07-23 修回日期:2010-10-19 出版日期:2011-06-10 发布日期:2011-05-10
  • 通讯作者: 牟颖 E-mail:ymu100@yahoo.com.cn
  • 基金资助:

    国家“九七三”计划项目(批准号: 2007CB714503, 2007CB714502), 国家科技部创新方法工作专项(批准号: 2008IM040800), 国家教育部博士学科点基金项目(批准号: 20090101110136 )和苏州市科技计划项目(批准号: ZXG0920)资助.

Surface Modification for Epoxy Resin Based Microfluidic Chip

WANG Lin-Lin1,2, HUANG Jiang1, JIN Wei1, JIN Qin-Han1, MU Ying1,2*   

  1. 1. Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Labaratory of Industrial Control Technology, Zhejiang University, Hangzhou 310058, China;
    2. Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun 130021, China
  • Received:2010-07-23 Revised:2010-10-19 Online:2011-06-10 Published:2011-05-10
  • Contact: MU Ying E-mail:ymu100@yahoo.com.cn
  • Supported by:

    国家“九七三”计划项目(批准号: 2007CB714503, 2007CB714502), 国家科技部创新方法工作专项(批准号: 2008IM040800), 国家教育部博士学科点基金项目(批准号: 20090101110136 )和苏州市科技计划项目(批准号: ZXG0920)资助.

摘要: 利用微流控芯片进行生物医学检测,往往需要对芯片通道进行表面修饰,连接上某些特异性的活性分子。本研究对一种新型的环氧树脂材料表面进行空气等离子体前处理,然后进行丙烯酸(acrylic acid, AAc)紫外诱导接枝聚合,使这种环氧树脂材料表面引入了羧基基团.在此基础上,将这种化学惰性的环氧树脂表面连接上特异的抗体,作为微流控芯片的基底,进行免疫检测实验。用静态接触角、甲苯胺兰染色、X射线光电子能谱分析(XPS)、BCA蛋白定量检测和细胞黏附实验等检测手段对环氧树脂的表面修饰进行了表征。结果表明,使用空气等离子体处理后丙烯酸紫外诱导接枝聚合的方法成功地在环氧树脂表面引入了大量的羧基基团;使用EDC/NHS偶联试剂,将蛋白以共价连接的方式连接到疏水的、化学惰性的环氧树脂表面;通过细胞黏附实验表明,用这种方法修饰的环氧树脂表面可以用作生物医学的免疫检测实验。

关键词: 环氧树脂, 表面修饰, 空气等离子体, 紫外光接枝聚合

Abstract: Microfluidic devices are potentially useful for biomedical detection, which sometimes need to be modified on the surface to immobilize active biomolecules. Surface modification of air-plasma-pretreated epoxy resin via near UV-induced graft polymerization with acrylic acid (AAc) was carried out. Specific antibodies were covalently immobilized on the surface of epoxy resin which were used to immunological detection. The modified epoxy resin were characterized by static water contact angle measurement, toluidine blue O staining test, X-ray photoelectron spectroscopy (XPS), bicinchoninic acid (BCA) test, and cell attachment. Our studies showed that the carboxyl groups were grafted onto the surface of epoxy resin, and covalently crosslinked with proteins. The results of cell attachment revealed that the modified epoxy resin could be applied to immunological detection.

Key words: Epoxy resin, Surface modification, Air plasma, UV-induced graft polymerization

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