高等学校化学学报

高等学校化学学报 ›› 2011, Vol. 32 ›› Issue (4): 957.

• 研究论文 • 上一篇    下一篇

含乳糖聚电解质靶向微胶囊的层层自组装构筑

高遐,张馥,陈志春,林贤福   

  1. 浙江大学化学系, 杭州 310027
  • 收稿日期:2010-07-23 修回日期:2010-09-13 出版日期:2011-04-10 发布日期:2011-03-09
  • 通讯作者: 林贤福 E-mail:llc123@zju.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 20874086)资助.

Hepatic-targeting Microcapsules Construction by Layer-by-layer Self-assembly of Lactose-branched Polyelectrolyte

GAO Xia, ZHANG Fu, CHEN Zhi-Chun, LIN Xian-Fu   

  1. Department of Chemistry, Zhejiang University, Hangzhou 310027, China
  • Received:2010-07-23 Revised:2010-09-13 Online:2011-04-10 Published:2011-03-09
  • Contact: LIN Xian-Fu E-mail:llc123@zju.edu.cn
  • Supported by:

    国家自然科学基金(批准号: 20874086)资助.

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被引次数

3

摘要： 设计合成了己二酸乳糖乙烯酯/对苯乙烯磺酸钠共聚物（PLESS），通过层层自组装技术构筑聚烯丙基胺盐酸盐（PAH）与含乳糖聚电解质PLESS的靶向微胶囊。以紫外-可见吸收光谱监测了PAH与PLESS在平面的石英片进行层层自组装过程，研究了不同实验条件（聚电解质浓度、溶液盐度、盐种类）对PAH/PLESS多层膜自组装的影响；PAH与PLESS在球形碳酸钙微球模板上层层自组装，去除模板后得到层状结构的微胶囊，用透射电镜（TEM）等方法观察其形态形貌；通过花生凝集素识别考察其潜在靶向性；通过细胞MTT活性试验评价其生物相容性。

关键词: 乳糖, 层层自组装, 微胶囊, 肝靶向性, 生物相容性

Abstract: Hepatic-targeting microcapsules were constructed by Layer-by-layer self-assembly of poly(allylamine hydrochloride) (PAH) and chemo-enzymatic synthesized poly(vinyl lactose ester-co-sodium p-styrenesulfonate) (PLESS) containing lactose branches. Alternate deposition of PAH and PLESS was carried out on different solid surfaces such as quartz slides and CaCO3 microparticles. The self-assembly nature of the novel polyelectrolytes was investigated in detail by varying polyelectrolytes concentration, ionic strength, and counteranion types. The assembly process and structure of multilayers were characterized by UV-vis. Hollow capsules were formed after the removal of CaCO3 templates and observed by TEM. The potential targetability of the system was proved in vitro by PNA lectin recognition. Furthermore biocompatibility of the polyelectrolytes was tested by MTT assay.

Key words: Lactose, Layer-by-layer Self-Assembly, Microcapsules, Hepatic targeting, Biocompatibility

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