高等学校化学学报

高等学校化学学报 ›› 2011, Vol. 32 ›› Issue (2): 322.

• 研究论文 • 上一篇    下一篇

亚微米级免疫磁球及其在细菌分离中的应用

牛牧1,杜美红2,邓奕1,2,高明远1   

  1. 1. 中国科学院化学研究所, 北京 100190;
    2. 北京市理化分析测试中心, 北京 100089
  • 收稿日期:2010-11-01 修回日期:2010-11-18 出版日期:2010-02-10 发布日期:2011-02-23
  • 通讯作者: 高明远 E-mail:gaomy@iccas.ac.cn
  • 基金资助:

    国家卫生部重大专项(批准号: 2008ZX10001-014)和中国科学院知识创新工程(批准号: KJCX2-YW-M15)资助.

Preparation and Application in Capturing Bacteria of Submicron Immunomagnetic Beads

NIU Mu1, DU Mei-Hong2, DENG Yi1,2, GAO Ming-Yuan1*   

  1. 1. Institue of Chemistry, Chinese Academy of Sciences, Beijing 100190, China;
    2. Beijing Center for Physical and Chemical Analysis, Beijing 100089, China
  • Received:2010-11-01 Revised:2010-11-18 Online:2010-02-10 Published:2011-02-23
  • Contact: GAO Ming-Yuan E-mail:gaomy@iccas.ac.cn
  • Supported by:

    国家卫生部重大专项(批准号: 2008ZX10001-014)和中国科学院知识创新工程(批准号: KJCX2-YW-M15)资助.

PDF (PC)

被引次数

26

摘要： 以亚微米级的单分散磁性微球为基础，制备出了表面包被有沙门氏菌抗体的免疫磁球. 利用表面电位、荧光和ELISA等方法研究了抗体在磁性微球表面的吸附行为. 在沙门氏菌磁分离实验中，通过调节投料抗体的浓度，研究了免疫磁球表面抗体浓度和磁分离效率的相关性，与微米级商品化免疫磁球的对比中，亚微米级的免疫磁球表现出了更高的磁分离效率.  

关键词: 亚微米, 免疫磁球, 沙门氏菌, 磁分离效率

Abstract: Based on the submicron monodispersed magnetic beads, the immunomagnetic beads covered with anti-Salmonella antibody were obtained. The adsorption behavior of IgG on the surface of magnetic beads was investigated through surface electric potential, fluorescence and ELISA method. In the immunoassay experiment, by adjusting the initial concentration of IgG, the relativity between surface concentration of IgG on magnetic beads and magnetic capture efficiency was also investigated. Finally, comparison with commercial Dynabeads, our submicron immunomagnetic beads represents higher capture efficiency for Salmonella.

Key words: submicron, immunomagnetic beads, Salmonella, magnetic capture efficiency

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