高等学校化学学报 ›› 2011, Vol. 32 ›› Issue (2): 275.

• 研究论文 • 上一篇    下一篇

醇/盐双水相体系中α-环己基扁桃酸手性分子识别

郭志峰,李芬芳,邢健敏   

  1. 中南大学化学化工学院, 长沙 410083
  • 收稿日期:2010-05-04 修回日期:2010-06-28 出版日期:2010-02-10 发布日期:2011-02-23
  • 通讯作者: 李芬芳 E-mail:lfflqq@mail.csu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:    20956001)和湖南省自然科学重点基金(批准号:   08jj3023)资助.

Chiral Recognition of α-Cyclohexyl-mandelic Acid in Alcohol/Salt-based Aqueous Two-phase Systems

GUO Zhi-Feng, LI Fen-Fang*, XING Jian-Min   

  1. School of Chemistry and Chemical Engineering,  Central South University,  Changsha 410083,  China
  • Received:2010-05-04 Revised:2010-06-28 Online:2010-02-10 Published:2011-02-23
  • Contact: LI Fen-Fang E-mail:lfflqq@mail.csu.edu.cn
  • Supported by:

    国家自然科学基金(批准号:    20956001)和湖南省自然科学重点基金(批准号:   08jj3023)资助.

摘要: 以含羟丙基-β-环糊精(HP-β-CD)手性识别剂的醇/盐双水相体系作为一种新型的手性识别萃取体系,研究了α-环己基扁桃酸(CHMA)对映体在其中的手性识别行为.详细考察了HP-β-CD浓度、CHMA浓度、乙醇和硫酸铵质量分数、体系温度和pH值等因素对CHMA对映体分配比(D)和分离因子(α)的影响.结果显示,含有手性识别剂HP-β-CD的乙醇∕硫酸铵双水相体系对CHMA对映体具有很强的手性识别能力;体系中HP-β-CD浓度、乙醇质量分数、温度和pH值等因素对对映体的分离度影响较大;在体系温度为40 ℃,pH值为2,乙醇质量分数为30%,硫酸铵质量分数为15%,HP-β-CD的浓度为50 g.L-1,CHMA浓度为0.5 mmol.L-1时,手性识别分离效果最佳,分离因子(α)达到了1.86.

关键词: 关键词 双水相体系, 手性识别, α-环己基扁桃酸对映体;羟丙基-β-环糊精

Abstract: A novel alcohol/salt-based aqueous two-phase system containing chiral selector hydroxypropyl-β-cyclodextrin(HP-β-CD) for enantioselective liquid-liquid extraction was introduced.The chiral recognition of α-cyclohexyl-mandelic acid(CHMA) enantiomers was studied in this system.The dependency of the distribution ratio (D) and the separation factors(α) on parameters such as the concentration of HP-β-CD,the concentration of CHMA, the mass fractions of C2H5OH and (NH4)2SO4,temperature and pH have been investigated.It was found that the C2H5OH-(NH4)2SO4-H2O aqueous two-phase system containing HP-β-CD as chiral selector showed a good chiral recognition ability toward CHMA enantiomers . It could be concluded that the enantioseparation strongly depended on the concentration of HP-β-CD,the mass fraction of C2H5OH,temperature and pH.The optimum conditions of enantioseparation were as follows:The temperature was 40 ℃,the pH was 2.0,the mass fraction of C2H5OH and (NH4)2SO4 was 30% and 15%, respectively,the concentration of HP-β-CD was 50 g.L-1,the concentration of CHMA was 0.5mmol.L-1.Under these conditions the separation factor(α) reached at 1.86.

Key words: Keywords Aqueous two-phase system, Chiral recognition, α-cyclohexyl-mandelic acid enantiomers;Hydroxypropyl-β-Cyclodextrin

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