高等学校化学学报 ›› 2010, Vol. 31 ›› Issue (9): 1712.

• 研究论文 • 上一篇    下一篇

微流控芯片中细胞动态胞吞二氧化硅纳米颗粒的研究

徐春秀1,2, 殷学锋1   

  1. 1. 浙江大学化学系, 微分析系统研究所, 杭州 310027;
    2. 韩山师范学院化学系, 潮州 521041
  • 收稿日期:2010-05-10 出版日期:2010-09-10 发布日期:2010-09-10
  • 通讯作者: 殷学锋, 男, 教授, 博士生导师, 主要从事微流控芯片分析研究. E-mail: yinxf@zju.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 20775072, 20890020)和浙江省自然科学基金(批准号: Z407029)资助.

Cellular Uptake of SiO2 Particles Under Dynamic Conditions in a Microfluidic Chip

XU Chun-Xiu1,2, YIN Xue-Feng1*   

  1. 1. Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310027, China;
    2. Department of Chemistry, Hanshan Normal University, Chaozhou 521041, China
  • Received:2010-05-10 Online:2010-09-10 Published:2010-09-10
  • Contact: YIN Xue-Feng. E-mail: yinxf@zju.edu.cn
  • Supported by:

    国家自然科学基金(批准号: 20775072, 20890020)和浙江省自然科学基金(批准号: Z407029)资助.

摘要: 研究了用微流控芯片在体外模拟人体血液流动状态下细胞胞吞二氧化硅纳米粒子的方法和特性. 通过调节储液池的液面差, 使细胞从微通道入口流入并在通道内沉积贴壁生长. 将含有贴壁细胞的微流控芯片放入37 ℃/体积分数5%CO2的培养箱中, 使细胞培养液连续流过贴壁细胞. 培养24 h后, 在流动的培养液中加入作为荧光标记物的500 nm 粒径的掺杂有异硫氰酸荧光素(FITC)的二氧化硅微球(MSN), 继续培养6 h后, 用荧光显微镜测定细胞胞吞二氧化硅纳米粒子后的荧光强度, 考察了不同流速下细胞对二氧化硅微球摄入量的影响. 结果表明, 在动态条件下, 细胞对二氧化硅微球的吞噬量明显下降, 当流速从0.022 mm/s 增加至0.74 mm/s时, 吞噬量从静态测得值的74.7%下降至7.1%.

关键词: 细胞胞吞, 微流控芯片, 纳米粒子, 二氧化硅微球

Abstract: An in vitro method based on microfludic chip was reported to investigate the cellular uptake of FITC-doped SiO2 nano-particles to simulate the in vivo blood flowing condition. Cell suspension was delivered from the inlet of the microchannel and immobilized onto the bottom of the channel in static conditions. The microfluidic chip containing adherent cell was placed inside an incubator(37 ℃ and 5% CO2 ). Culture medium is continuously transported through the microchannel by adjusting the liquid levels of the reservoirs for one day. After culturing the cells inside microfluidic channel, FITC-doped SiO2 particles with a diameter of 500 nm as fluorescent markers were added to the culture medium, and perfused through the microchannel via the adhered cells at different flow rates for 6 h. The effect of flow rate on the uptake efficiency of FITC-doped SiO2 particles was determined by fluorescence microscope. Compared to the result(100%) in conventional cell culture flask, the uptake efficiency was significantly decreased from 74.7% to 7.1%, when the flow rate increased from 0.022 mm/s to 0.74 mm/s.

Key words: Cellular uptake, Microchip, Nanoparticle, Silicon dioxide particle

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