高等学校化学学报

高等学校化学学报 ›› 2010, Vol. 31 ›› Issue (12): 2322.

• 研究论文 • 上一篇    下一篇

纳米ZnO对白僵菌孢子的紫外保护及与孢子的生物相容性

陈培荣1,2,王滨1,姚洪章1,林华峰3,黄勃1,李增智1   

  1. 1. 安徽农业大学微生物防治安徽省重点实验室, 合肥 230036
    2. 安徽农业大学微理学院应用化学系, 合肥 230036
    3. 安徽农业大学植物保护学院, 合肥 230036
  • 收稿日期:2010-06-30 修回日期:2010-09-14 出版日期:2010-12-10 发布日期:2010-12-06
  • 通讯作者: 李增智 E-mail:zzllizz@163.com
  • 作者简介:李增智, 男, 教授, 博士生导师, 主要从事害虫生物防治的研究. E-mail: zzli@ahau.edu.cn
  • 基金资助:

    国家“八六三”计划项目(批准号: 2006AA10A212)、 国家自然科学基金(批准号: 30972368)和安徽省教育厅科研项目(批准号: KJ2007B232)资助.

Protection of ZnO Nanoparticles to Beauveria bassiana Conidia from Ultraviolet Radiation and Their Biocompatibility

CHEN PeiRong1,2,WANG Bin1,YAO HongZhang1,LIN HuaFeng3,HUANG Bo1,LI ZengZhi1*   

  1. 1. Anhui Provincial Key Laboratory of Microbial Control, Anhui Agricultural University, Hefei 230036, China
    2. Department of Applied Chemistry, School of Science, Anhui Agricultural University, Hefei 230036, China
    3. College of Plant Protection, Anhui Agricultural University, Hefei 230036, China
  • Received:2010-06-30 Revised:2010-09-14 Online:2010-12-10 Published:2010-12-06
  • Contact: LI ZengZhi E-mail:zzllizz@163.com
  • About author:李增智, 男, 教授, 博士生导师, 主要从事害虫生物防治的研究. E-mail: zzli@ahau.edu.cn
  • Supported by:

    国家“八六三”计划项目(批准号: 2006AA10A212)、 国家自然科学基金(批准号: 30972368)和安徽省教育厅科研项目(批准号: KJ2007B232)资助.

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被引次数

9

摘要： 用沉淀法制备经油酸表面修饰的纳米ZnO，测定其紫外吸收性能及对油悬浮剂型白僵菌孢子的抗紫外保护效率；用扫描电子显微镜观察纳米ZnO与孢子的混合状态，通过对含纳米ZnO的白僵菌制剂在贮存期间孢子萌发率差异显著性的分析，确定其与白僵菌孢子的生物相容性.结果表明：制备的纳米ZnO的平均粒径为27nm，其对波长200nm～370nm范围的紫外光具有良好的吸收性能.含纳米ZnO质量分数为1.0%、2.0%和5.0%的油悬浮剂型白僵菌，紫外灯照射8h，对孢子保护效率均大于60%，不含纳米ZnO为9.7％. 纳米ZnO被吸附包覆于孢子表面，屏蔽紫外线，保护孢子免受紫外线的杀伤.含0.5%、1.0%和2.0%纳米ZnO的油悬浮剂型白僵菌制剂室温贮存10个月，其孢子萌发率与不含纳米ZnO的差异不显著；纳米ZnO与白僵菌孢子生物相容性好.油悬浮剂型白僵菌制剂中纳米ZnO的适宜含量为1.0%～2.0%.

关键词: 纳米ZnO, 白僵菌, 抗紫外剂, 生物相容性

Abstract: ZnO nanoparticles modified with oleic acid were prepared by chemical precipitation method, and their ultraviolet absorption properties and protection efficiency to Beauveria bassiana conidia in oil formulation were investigated. The mixture of ZnO nanoparticles and conidia were observed with SEM, and their biocompatibility was evaluated, based on significant difference levels which were analyzed by measuring germination percentage of conidia in formulation after storage. The results show that the obtained ZnO nanoparticles with particles size of 27nm had a strong absorption in the 200-370nm ultraviolet wavelength range. After 8-h exposure to xenon light，the protection efficiency of ZnO nanoparticles to conidia in oil formulation with 1.0% (mass fraction), 2.0% and 5.0% ZnO nanoparticles were over 60%, while the control group’s protection efficiency was 9.7%. A mass fraction of ZnO nanoparticles were absorbed on the surface of conidia and a coating layer that provided protection to conidia from UV degradation was formed. After 10-month room storage, there were no significant differences for conidia germination among four oil formulations containing 0.0%, 0.5%, 1.0% and 2.0% ZnO nanoparticles. As a result, ZnO nanoparticles had a good biocompatibility with Beauveria bassiana. The concentration of ZnO nanoparticles added to conidia oil formulation should be 1.0%-2.0%.

Key words: ZnO nanoparticle, Beauveria bassiana, UV protection agent, Biocompatibility

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