双龙方组分诱导大鼠BMSCs分化的差异基因筛选及聚类分析

高等学校化学学报 ›› 2009, Vol. 30 ›› Issue (9): 1729.

双龙方组分诱导大鼠BMSCs分化的差异基因筛选及聚类分析

范雪梅¹, 李雪², 梁琼麟³, 胡坪⁴, 王义明³, 罗国安^1,3

1. 华东理工大学药学院, 上海 200237;
2. 清华大学医学院,
3. 化学系, 北京 100084;
4. 华东理工大学化学与分子工程学院, 上海 200237

收稿日期:2008-09-12 出版日期:2009-09-10 发布日期:2009-09-10
通讯作者: 罗国安, 男, 教授, 博士生导师, 主要从事中药现代化相关课题研究. E-mail: luoga@tsinghua.edu.cn
基金资助:
科学技术部“十一五”科技支撑计划(批准号: 2006BA108B04-01)和国家“九七三”计划(批准号: 2005CB523503)资助.

Screening and Cluster of Differentially Expressed Gene of MSCs Induced by Shuanglong Prescription Components

FAN Xue-Mei¹, LI Xue², LIANG Qiong-Lin³, HU Ping⁴, WANG Yi-Ming³, LUO Guo-An^1,3*

1. School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China;
2. School of Medicine,
3. Department of Chemistry, Tsinghua University, Beijing 100084, China;
4. School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China

Received:2008-09-12 Online:2009-09-10 Published:2009-09-10
Contact: LUO Guo-An. E-mail: luoga@tsinghua.edu.cn
Supported by:
科学技术部“十一五”科技支撑计划(批准号: 2006BA108B04-01)和国家“九七三”计划(批准号: 2005CB523503)资助.

摘要/Abstract

摘要：

利用基因芯片筛选双龙方有效组分(总人参皂苷及总丹酚酸)诱导大鼠骨髓间充质干细胞(BMSCs)类心肌细胞分化过程中的差异表达基因, 并对其进行聚类分析, 在基因水平研究了双龙方组分对大鼠BMSCs分化的影响. 对大鼠BMSCs进行分组培养, 分别收集10, 20, 30及40 d的细胞样本, 提取tRNA, 经基因芯片检测, 筛选出BMSCs变化过程中的差异表达基因并进行生物信息学分析, 同时通过差异表达基因对样本进行Hierarchical聚类分析. 在BMSCs的分化过程中, 筛选出179条差异表达基因, 经分析发现它们与能量代谢和信号传导等多类基因密切相关. 对样本进行聚类分析发现其聚为两大类: 10和20 d的样本聚为一类, 30和40 d的样本聚为一类. 说明BMSCs在20~30 d之间可能发生了显著的改变.

关键词: 双龙方; 基因芯片; 差异表达基因; 聚类分析

Abstract:

To investigate the effects of Shuanglong prescription components and the differential genes on the stem cells differentiation into cardiomyocytes via cDNA microarray, the differentially expressed genes were recorded by cluster analysis. We divided and separately cultured the Rat BMSCs, collected the cell samples on 10, 20, 30 and 40 d, then distilled total RNA. Microarray was used to detect the differentially expressed genes between each group. The differential expressed genes were screened and analysed by cluster. We got 179 differentially expressed genes during the differentiation. By bioinformal analysis, these genes were associated with metabolism, signal transduction etc. Using hierarchical method, we found that 10 and 20 d samples were clustered in one class, 30 and 40 d samples were clustered in another class. It illuminated that BMSCs after induced may generated distinctly change between 20 and 30 d. Shuanglong prescription components may induced BMSCs differentiated into cardiomyocyte-like cells.

Key words: Shuanglong prescription; Gene chip; Differentially expressed gene; Cluster analysis

TrendMD:

范雪梅, 李雪, 梁琼麟, 胡坪, 王义明, 罗国安. 双龙方组分诱导大鼠BMSCs分化的差异基因筛选及聚类分析. 高等学校化学学报, 2009, 30(9): 1729.

FAN Xue-Mei, LI Xue, LIANG Qiong-Lin, HU Ping, WANG Yi-Ming, LUO Guo-An*. Screening and Cluster of Differentially Expressed Gene of MSCs Induced by Shuanglong Prescription Components. Chem. J. Chinese Universities, 2009, 30(9): 1729.

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