水溶性羟基化单壁碳纳米管与人血清白蛋白之间的相互作用研究

高等学校化学学报 ›› 2009, Vol. 30 ›› Issue (9): 1733.

水溶性羟基化单壁碳纳米管与人血清白蛋白之间的相互作用研究

刘淑芳, 尹俊发, 宋茂勇, 汪海林

中国科学院生态环境研究中心, 环境化学与生态毒理学国家重点实验室, 北京 100085

收稿日期:2008-09-01 出版日期:2009-09-10 发布日期:2009-09-10
通讯作者: 汪海林, 男, 博士, 研究员, 主要从事生物分析和分子毒理学研究. E-mail: hlwang@rcees.ac.cn
基金资助:
中国科学院知识创新工程青年人才领域前沿项目(批准号: RCEES-QN-200715)资助.

Interaction Between Water-soluble Hydroxylated Single-wall Carbon Nanotubes and Human Serum Albumin

LIU Shu-Fang, YIN Jun-Fa, SONG Mao-Yong, WANG Hai-Lin*

State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 10085, China

Received:2008-09-01 Online:2009-09-10 Published:2009-09-10
Contact: WANG Hai-Lin. E-mail: hlwang@rcees.ac.cn
Supported by:
中国科学院知识创新工程青年人才领域前沿项目(批准号: RCEES-QN-200715)资助.

摘要/Abstract

摘要：

利用荧光光谱、UV吸收光谱、同步荧光光谱和透射电子显微镜等技术较为系统地研究了水溶性羟基化单壁碳纳米管与人血清白蛋白(HSA)的相互作用. 实验发现, 这种羟基化单壁碳纳米管可以明显猝灭HSA的内源荧光, 且猝灭效应随碳纳米管浓度增大而增强. 同时, HSA对水溶性羟基化单壁碳纳米管起到一定的分散和稳定作用. 同步荧光光谱表明, 二者之间的相互作用可导致HSA的构象发生变化, HSA的色氨酸残基荧光信号发生2 nm的红移, 表明色氨酸残基周围微环境的极性降低.

关键词: 碳纳米管; 人血清白蛋白; 透射电子显微镜; 荧光; 同步荧光; 荧光猝灭

Abstract:

The interaction between water-soluble hydroxylated single-wall carbon nanotube and human serum albumin and other related effects were investigated using fluorescence spectroscopy, absorption spectroscopy, synchronous fluorescence spectroscopy and transmission electron microscopy(TEM) methods. Fluorescence quenching of human serum albumin by a hydroxylated single-wall carbon nanotube was observed, indicating the interaction between them occurs. TEM images show the hydroxylated carbon nanotubes system can be stabilized and dispersed by the absorption of human serum albumin on the surface of the hydroxylated single-wall carbon nanotubes. The synchronous fluorescence spectra of human serum albumin indicate the slight change of HSA conformation and the microenvironment of tryptophan residues in human serum albumin. The experimental results may provide us some important information for the understanding of transportation of hydroxylated single-wall carbon nanotubes and other related biological effects.

Key words: Carbon nanotubes; Human serum albumin; Transmission electron microscopy(TEM); Fluorescence; Synchronous fluorescence; Fluorescence quenching

TrendMD:

刘淑芳, 尹俊发, 宋茂勇, 汪海林. 水溶性羟基化单壁碳纳米管与人血清白蛋白之间的相互作用研究. 高等学校化学学报, 2009, 30(9): 1733.

LIU Shu-Fang, YIN Jun-Fa, SONG Mao-Yong, WANG Hai-Lin*. Interaction Between Water-soluble Hydroxylated Single-wall Carbon Nanotubes and Human Serum Albumin. Chem. J. Chinese Universities, 2009, 30(9): 1733.

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