预处理对丝素蛋白膜调控羟基磷灰石晶体生长的影响

高等学校化学学报 ›› 2009, Vol. 30 ›› Issue (10): 1987.

预处理对丝素蛋白膜调控羟基磷灰石晶体生长的影响

刘琳¹, 孔祥东², 李玉成¹, 蔡玉荣¹, 姚菊明¹

1. 浙江理工大学材料与纺织学院, 教育部先进纺织材料与制备技术重点实验室,
2. 生命科学学院, 杭州 310018

收稿日期:2009-03-05 出版日期:2009-10-10 发布日期:2009-10-10
通讯作者: 姚菊明, 男, 博士, 教授, 主要从事生物高分子研究. E-mail: yaoj@zstu.edu.cn
基金资助:
长江学者和创新团队发展计划(批准号: IRT0654)、新世纪优秀人才支持计划(批准号: NCET070763)、国家“九七三”计划前期研究专项(批准号: 2008CB617506)和钱江人才计划(批准号: 2008R10006)资助.

Effect of Pretreated Silk Fibroin Films on the Regulation of Hydroxyapatite Crystal Growth

LIU Lin¹, KONG Xiang-Dong², LI Yu-Cheng¹, CAI Yu-Rong¹, YAO Ju-Ming^1*

1. Key Laboratory of Advanced Textile Materials and Manufacturing Technology, Ministry of Education, College of Materials and Textile,
2. College of Life Science, Zhejiang Sci-Tech University, Hangzhou 310018, China

Received:2009-03-05 Online:2009-10-10 Published:2009-10-10
Contact: YAO Ju-Ming. E-mail: yaoj@zstu.edu.cn
Supported by:
长江学者和创新团队发展计划(批准号: IRT0654)、新世纪优秀人才支持计划(批准号: NCET070763)、国家“九七三”计划前期研究专项(批准号: 2008CB617506)和钱江人才计划(批准号: 2008R10006)资助.

摘要/Abstract

摘要：

以丝素蛋白膜为基质, 在模拟体液中诱导羟基磷灰石晶体在其表面沉积和生长. 利用XRD, SEM, HRTEM, AFM和FTIR等表征手段研究了不同预处理方法对羟基磷灰石晶体的形成及其微观形貌的影响. 结果表明, 丝素膜可有效地诱导羟基磷灰石晶体在其表面沉积和生长; 较长的矿化时间有利于形成较多结晶度良好的HAP晶体; 而不同预处理方法对丝素膜的表面结构产生了不同影响, 进而调制在其表面沉积的羟基磷灰石的形貌和生长方向. 同时对丝素蛋白膜调控羟基磷灰石晶体生长的机制进行了必要的探讨.

关键词: 生物矿化; 丝素蛋白膜; 羟基磷灰石; 模拟体液

Abstract:

The silk fibroin films were used as the organic templates to induce the deposition of hydroxyapatite(HAP) crystals in the simulate body fluid. The effect of deposition time and different pretreatment methods for silk fibroin film on the formation of HAP crystals was investigated via XRD, SEM, HRTEM, AFM, FTIR, etc. The results indicate that the silk fibroin films can regulate effectively the deposition and growth of HAP crystals. Longer deposition time is apt to form more HAP crystals with well crystallinity. Furthermore, diffe-rent pretreatment methods lead to various morphology and growth direction of HAP crystals according to the rearrangement of silk film′s surface groups and activation of carboxyl and amino groups induce by EDC/NHS. The mechanism for the silk fibroin film regulating the HAP crystal growth was discussed. The study on the effect of pretreated silk fibroin films on the regulation of hydroxyapatite crystal growth will be helpful for further understanding the process of silk fibroin biomineralization.

Key words: Biomineralization; Silk fibroin film; Hydroxyapatite; Simulated body fluid

TrendMD:

刘琳, 孔祥东, 李玉成, 蔡玉荣, 姚菊明. 预处理对丝素蛋白膜调控羟基磷灰石晶体生长的影响. 高等学校化学学报, 2009, 30(10): 1987.

LIU Lin, KONG Xiang-Dong, LI Yu-Cheng, CAI Yu-Rong, YAO Ju-Ming*. Effect of Pretreated Silk Fibroin Films on the Regulation of Hydroxyapatite Crystal Growth. Chem. J. Chinese Universities, 2009, 30(10): 1987.