高等学校化学学报

高等学校化学学报 ›› 2009, Vol. 30 ›› Issue (10): 1980.

• 研究论文 • 上一篇    下一篇

金乌贼脑和视神经节蛋白质组比较分析

黄福生1, 陈海滨1, 黄琳1, 黄河清1,2,3   

  1. 1. 厦门大学生命科学学院生物化学与生物技术学系,
    2. 近海海洋环境科学国家重点实验室, 海洋与环境学院,
    3. 化学化工学院化学生物学福建省重点实验室, 厦门 361005
  • 收稿日期:2009-06-23 出版日期:2009-10-10 发布日期:2009-10-10
  • 通讯作者: 黄河清, 男, 教授, 博士生导师, 主要从事蛋白质结构与功能及蛋白质组学研究. E-mail: hqhuang@xmu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号： 04776060)和福建省高校创新团队资金资助.

Comparison Analysis of Proteome of Both Cerebral and Optic Ganalions in Sepia esculenta

HUANG Fu-Sheng1, CHEN Hai-Bin1, HUANG Lin1, HUANG He-Qing1,2,3*   

  1. 1. Department of Biochemistry and Biotechnology, School of Life Sciences,
    2. State Key Laboratory of Marine Environmental Science, College of Oceanography and Environmental Science,
    3. Key Laboratory of Chemical Biology of Fujian Province, College of Chemistry & Chemical Engineering, Xiamen University, Xiamen 361005, China
  • Received:2009-06-23 Online:2009-10-10 Published:2009-10-10
  • Contact: HUANG He-Qing. E-mail: hqhuang@xmu.edu.cn
  • Supported by:

    国家自然科学基金(批准号： 04776060)和福建省高校创新团队资金资助.

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摘要：

采用双向凝胶电泳(2D-PAGE)技术优化分离金乌贼的脑及视神经节全蛋白质, 并选用肽质量指纹谱(Peptide mass fingerprinting, PMF)技术和数据库检索方法对2D-PAGE图谱上的部分蛋白质斑点进行鉴定, 初步构建了金乌贼视神经节(Optic ganglion of Sepia esculenta, SEOG)和脑神经节(Cerebral ganglion of Sepia esculenta, SECG)部分分子解剖图谱. 用Melanie 4 Trial软件分析脑神经节和视神经节蛋白质斑点总数量分别为682和594个, 其中SECG蛋白质斑点数量明显多于SEOG. 在脑神经节和视神经节中均发现了线粒体苹果酸脱氢酶前体(Mitochondrial malate dehydrogenase precursor, pre-MDH)及可溶性NSF连接蛋白(SNAP-type proteins). 此外, 延长因子(Elongation factor G)、微管蛋白(Tubulin)和肌动蛋白(Actin)等蛋白质也具有高匹配率. 已鉴定的蛋白质, 多数归属于假定蛋白和结构蛋白类.

关键词: 金乌贼; 脑神经节; 视神经节; 蛋白质组学; 鉴定

Abstract:

Sepia esculenta vests in a cephalopoda animal in mollusca. The differentitation of neural system show not only relative higher level in Cephalopoda animal, but also is easily to separate for studying in the neural sciences. Here, proteomes of both cerebral and optic ganglions in Sepia esculenta were effectively separated by a improved approach of 2D-PAGE. In addition, we used both peptide mass fingerprinting(PMF) and database search to identify protein spots in part in 2D-PAGE gel for establishing maps of molecular anatomic of cerebral and optic ganglions primarily in Sepia esculenta. We used a software of Melanie 4 Trial to analyze those ganglions, indicating approximately 682 and 594 protein spots in cerebral and optic ganglions, respectively, which the spot numbers in front more than that in later. Moreover, the same proteins such as mitochondrial malate dehydrogenase precursor, pre-MDH, SNAP-type proteins can be found by both ganglions. In addition, these spots such as elongation factor G, tubulin, and actin shows high match rate, but most protein identified in the gel are hypothetic and structural proteins.

Key words: Sepia esculenta; Cerebral ganglion; Optic ganglion; Proteomics; Identification

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