高等学校化学学报 ›› 2006, Vol. 27 ›› Issue (4): 608-611.

• 研究论文 • 上一篇    下一篇

应用分子印迹-固相萃取法提取中药活性成分非瑟酮

李礼1, 胡树国1, 何锡文1, 李文友1, 陈朗星1,   张玉奎1,2   

  1. 1. 南开大学化学学院, 天津 300071;  2. 中国科学院大连化学物理研究所, 大连 116011
  • 收稿日期:2005-06-02 出版日期:2006-04-10 发布日期:2006-04-10
  • 通讯作者: 张玉奎(1942年出生), 男, 研究员, 博士生导师, 中国科学院院士, 从事色谱基础理论及新技术开发应用研究. E-mail: Ykzhang@online.in.cn
  • 基金资助:

    国家自然科学基金(批准号: 20375017, 20375019和20445001)和天津市自然科学基金(批准号: 033603511)资助

Solid-phase Extraction of Active Component of  Chinese Traditional Medicine  Fisetin by Using Molecularly Imprinted Polymers

LI Li 1, HU Shu-Guo1, HE Xi-Wen1*, LI Wen-You1, CHEN Lang-Xing1, ZHANG Yu-Kui 1,2*   

  1. 1.  College of Chemistry, Nankai University, Tianjin 300071, China;
    2. Dalian Institute of Chemical Physics, Chinese Academy  of Sciences, Dalian 116023, China
  • Received:2005-06-02 Online:2006-04-10 Published:2006-04-10
  • Contact: HE Xi-Wen1,xiwenhe@nankai.edu.cn; ZHANG Yu-Kui, E-mail: Ykzhang@online.in.cn

摘要:

分别以中药黄栌的主要成分非瑟酮为印迹分子、 丙烯酰胺为功能单体及乙二醇二甲基丙烯酸酯为交联剂, 通过封管聚合法合成了分子印迹聚合物; 将其装于自制的固相萃取柱中, 研究了以不同体积比的乙醇-水溶液为溶剂时非瑟酮在柱上的保留行为; 通过优化清洗及洗脱条件, 使非瑟酮与它的结构相似物槲皮素在柱上得到了很好的分离.

关键词: 非瑟酮; 槲皮素; 分子印迹聚合物(MIPs); 固相萃取; 中药

Abstract:

Fisetin is the main function integrant in Cotinus coggygria scop of Chinese traditional medicine. Using fisetin as imprinted molecule, acrylamide as the functional monomer, and EDMA as the  crosslinking agent, a polymer was prepared by molecular imprinting technique. The polymer was loaded into the self-made solid-phase extraction(SPE) cartridge. A SPE procedure was suggested for extracting and concentrating fisetin by  using different volume fractions of ethanol in aqueous solution as the loading conditions. In order to optimize the washing and eluting solution, the adsorption of fisetin in different solutions was studied. It was found that the fisetin and analogue quercetin were separated primely.

Key words: Fisetin; Quercetin;  Molecularly imprinted polymers(MIPs); Solid-phase extraction; Chinese traditional medicine

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