高等学校化学学报

高等学校化学学报 ›› 2006, Vol. 27 ›› Issue (3): 485.

• 研究简报 • 上一篇    下一篇

系列甘露糖醛酸寡糖的制备与鉴定

刘斌1,王长云1,张洪荣1,王斌贵2,魏玉西3,康凯1,李亮1,于广利1,耿美玉1,管华诗1   

  1. 1. 中国海洋大学海洋药物研究所,海洋药物教育部重点实验室,青岛 266003;
    2. 中国科学院海洋研究所实验海洋生物学重点实验室,青岛 266071;
    3. 青岛大学生物系,青岛 266071
  • 收稿日期:2005-03-24 出版日期:2006-03-10 发布日期:2006-03-10
  • 通讯作者: 王长云(1965年出生),男,博士,教授,博士生导师,从事海洋药物化学研究. E-mail: changyun@ouc.edu.cn
  • 基金资助:

    国家“九七三”计划(批准号: 2003CB716400)、 国家“八六三”计划(批准号: 2004AA621020)、 山东省优秀中青年科学家科研奖励基金(批准号: 03BS109)和中国科学院海洋研究所实验海洋生物学重点实验室开放基金(2004)资助.

Preparation and Identification of Series of Oligomannuronates

LIU Bin1,WANG Chang-Yun1*,ZHANG Hong-Rong1,WANG Bin-Gui2,WEI Yu-Xi3,KANG Kai1,LI Liang1,YU Guang-Li1,GENG Mei-Yu1,GUAN Hua-Shi1   

  1. 1. Key Laboratory of Marine Drugs,Ministry of Education,Institute of Marine Drugs,Ocean University of China,Qingdao 266003,China;2. Laboratory of Experimental Marine Biology,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China;
     3. Department of Biology,Qingdao University,Qingdao 266071,China
  • Received:2005-03-24 Online:2006-03-10 Published:2006-03-10

PDF (PC)

被引次数

19

摘要：

用酸降解法制备了系列甘露糖醛酸寡糖(聚合度2～8),并分析测定了寡糖的结构. 褐藻胶经部分酸水解,于pH=2.85处分级获得聚甘露糖醛酸. 继续用酸降解法降解聚甘露糖醛酸,经凝胶柱层析分离纯化,获得系列甘露糖醛酸寡糖. 用荧光标记糖电泳(FACE)对寡糖进行了分析,并用电喷雾离子化质谱(ESI-MS)、 核磁共振波谱(NMR)及红外光谱(FTIR)进行了结构表征.
本研究用酸降解法制备饱和甘露糖醛酸寡糖,用凝胶柱层析法分离获得系列聚合度的寡糖,为褐藻胶大分子构效关系研究和药物的筛选与发现提供了重要的基础资料.

关键词: 褐藻胶; 酸降解; 甘露糖醛酸寡糖; 制备; 鉴定

Abstract:

A series of oligomannuronates with series degree of polymerization(DP=2-8) were prepared by acid hydrolysis from alginate-derived homopolymannuronate blocks,and the structures of the oligosaccharides were analyzed. The macromolecule of alginate was treated by partial acid hydrolysis and fractionated at pH=2.85 resulting in homopolymannuronate blocks. The homopolymer was degraded by acid hydrolysis,and the oligosaccharides were separated and purified by gel chromatography. The oligosaccharides were characterized by fluorophore-assisted carbohydrate electrophoresis(FACE),and the structures of derived oligomannuronates were determined by ESI-MS,NMR as well as FTIR analysis.

Key words: Alginate; Acid hydrolysis; Oligomannuronate; Preparation;Identification

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