高等学校化学学报 ›› 2006, Vol. 27 ›› Issue (1): 79-81.

• 研究快报 • 上一篇    下一篇

苯肾上腺素诱导乳鼠心肌细胞蛋白质
表达变化的蛋白质组分析

刘宁1, 李子健2, 刘志强1, 刘淑莹1, 韩启德2, 张幼怡2   

  1. 1. 中国科学院长春应用化学研究所新药研究实验室, 长春质谱中心, 长春 130022;
    2. 北京大学第三医院血管医学研究所, 分子心血管学教育部重点实验室, 北京 100083
  • 收稿日期:2005-10-08 出版日期:2006-01-10 发布日期:2006-01-10
  • 通讯作者: 刘志强(1962年出生), 男, 博士, 研究员, 博士生导师, 主要从事质谱分析研究. E-mail: liuzq@ciac.jl.cn 张幼怡(1956年出生), 女, 博士, 研究员, 博士生导师, 从事心血管受体研究. E-mail: zhangyy@bjmu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 20273067, 30400552)、 中国科学院知识创新工程重要方向项目(批准号: KGCX2-SW-213-06)及国家重点基础研究发展规划项目(“九七三”计划)(批准号: G2000056906)资助

Proteomic Analysis of Proteins with Altered Expression Induced
by Phenylephrine in Neonatal Rat Cardiomyocyte

LIU Ning1,  LI Zi-Jian 2,  LIU Zhi-Qiang1,  LIU Shu-Ying2,  HAN Qi-De1,  ZHANG You-Yi2   

  1. 1.  Laboratory of New Drug Research and Mass Spectrometry,  Changchun Institute of Applied Chemistry, 
    Chinese Academy of Sciences,  Changchun Center of Mass Spectrometry,  Changchun 130022,    China;
    2.  Institute of Vascular Medicine,  Third Hospital, Key Laboratory of
    Molecular Cardiovascular Sciences of  Ministry of Education,  Peking University, Beijing 100083,   China
  • Received:2005-10-08 Online:2006-01-10 Published:2006-01-10
  • Contact: LIU Zhi-Qiang,E-mail:liuzq@ciac.jl.cn ZHANG You-Yi,E-mail:zhangyy@bjmu.edu.cn

摘要:

α1-肾上腺素受体(α1-Adrenergic receptor, α1-AR)是G蛋白偶联受体(G-protein coupled receptor, GPCR), 也是内源性儿茶酚胺、 去甲肾上腺素和肾上腺素最重要的靶受体之一. α1-AR广泛分布于机体的各种器官、 组织和细胞中, 并介导多种生理效应, 如血管收缩、 蛋白质合成及心脏变力变时作用等[1,2]. 很多研究已经证实, α1-AR及其信号转导通路与许多心血管疾病存在密切关系[3,4]. 蛋白质组学可提供一种发现在疾病情况下异常表达蛋白质的方法, 为疾病的早期诊断和愈后判断提供指南, 并为针对性疾病治疗提供科学依据

关键词: 苯肾上腺素; 心肌细胞; 蛋白质组

Abstract:

AbstractIn order to find candidate proteins that are potentially associated with the phenotype in neonatal rat cardiomyocytes treated with phenylephrine(PE),   the differential proteins expression pattern after α-adrenergic receptor stimulation in neonatal cardiomyocytes was analyzed by  using a series of methods,  including two dimensional polyacrylamide gel electrophoresis,  PDQuest 2-DE software analysis and peptide mass fingerprint mapping based on MALDI-TOF-MS.  The good two-dimensional  pattern with a high resolution and reproducibility was obtained.  Totally 14 differential protein spots were found.  Out of these,  11 proteins were preliminarily identified. Desmin, Txndc5, etc. were found to be up-regulated in PE-treated cardiomyocytes, while caspase-11, 4-hydroxyphenylpyruvic acid dioxygenase, etc. were found to be down-regulated. It suggests that the differential expression analysis of proteome may be useful to the further study of the related proteins and the molecular markers of cardiovascular diseases.

Key words: Phenylephrine; Cardiomyocyte; Proteome

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