高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (9): 1634.

• 研究快报 • 上一篇    下一篇

基于银增强金标记物的阳极溶出伏安免疫分析用于补体第三成分的测定

楚霞1,2, 傅昕1, 沈国励2, 俞汝勤2   

  1. 1. 湖南师范大学化学化工学院,长沙410081;
    2. 湖南大学化学与生物传感及化学计量学国家重点实验室,长沙410082
  • 收稿日期:2004-09-29 出版日期:2005-09-10 发布日期:2005-09-10
  • 基金资助:

    国家自然科学基金(批准号:20105007)资助.

Anodic Stripping Voltammetric Immunoassay Based on Silver Enhanced Gold Label for Determination of Complement Ⅲ

CHU Xia1,2, FU Xin1, SHEN Guo-Li2, YU Ru-Qin2   

  1. 1. Chemistry and Chemical Engineering College, Hunan Normal University, Changsha 410081, China;
    2. State Key Laboratory for Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, China
  • Received:2004-09-29 Online:2005-09-10 Published:2005-09-10

关键词: 金属免疫分析, 阳极溶出伏安法, 银增强溶液, 免疫金标记物

Abstract: A novel, sensitive anodic stripping volammetric immunoassay was developed based on silver-enhanced immuno-gold label. The immunoreaction was performed in a polystyrene microwell by using the sandwich format. Primary antibodies specific for C3(complement Ⅲ) were adsorbed passively on the walls of a polystyrene microwell. The C3 analyte was first captured by the primary antibody and then sandwiched by a secondary colloidal gold-labeled antibody. The addition of the silver enhancement solution results in the precipitation of a large amount of silver on colloidal gold labels due to the catalytic reduction which, after silver metal dissolution in an acidic solution, was determined by anodic stripping voltammetry(ASV) at a glassy-carbon electrode. The influence of some immunoassay conditions upon the anodic stripping peak current was examined and optimized. The anodic stripping peak current depended linearly on the logarithm of C3 mass concentration over the range of 7.2 ng/mL to 7.33 μg/mL and a detection limit as low as 7ng/mL is achieved. The anodic stripping volammetric immunoassay was applied to the determination of C3 concentration in human serum with satisfactory results.

Key words: Metalloimmunoassay, Anodic stripping voltammetry, Silver enhancement solution, Immuno-gold label

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