高等学校化学学报

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (8): 1446.

• 研究论文 • 上一篇    下一篇

柴胡逆转肝细胞癌多药耐药作用与相关机制的研究

盖晓东1, 曾常茜1, 洪敏2   

  1. 1. 北华大学生命科学与生物技术实验室, 吉林132013;
    2. 吉林大学医学蛋白质与蛋白质组学实验室, 长春130023
  • 收稿日期:2004-10-29 出版日期:2005-08-10 发布日期:2005-08-10
  • 通讯作者: 盖晓东(1962年出生),女,博士,教授,从事肿瘤生物学研究.E-mail:gaixiaodong@yahoo.com.cn E-mail:gaixiaodong@yahoo.com.cn
  • 基金资助:

    吉林省教育厅科研项目(批准号:200026);吉林省卫生厅科研项目(批准号:200104)资助.

Effect of Bupleurun Chinese DC(BCDC) on the MDR Reversal of Hepatocellular Carcinoma and Related Mechanism

GAI Xiao-Dong1, ZENG Chang-Qian1, HONG Min2   

  1. 1. Laboratory of Life Science and Biological Technology, Beihua University, Jilin 132013, China;
    2. Medicine Protein and Proteomics laboratory, Jilin University, Changchun 130023, China
  • Received:2004-10-29 Online:2005-08-10 Published:2005-08-10

PDF (PC)

被引次数

48

摘要： 选用对VCR天然耐药肝癌细胞株Bel-7402为对象来研究柴胡逆转MDR效果与相关的机制,以寻找一种新型的具有多药耐药逆转活性的中药.结果表明,柴胡具有逆转肝细胞癌多药耐药作用,并与抗癌药物VCR有协同作用.柴胡对人肝癌Bel-7402细胞株具有多药耐药逆转作用,提高了耐药细胞内的VCR含量,增加了VCR对细胞G2期阻滞作用,抑制了P-170糖蛋白的表达,抑制MDR1/mRNA的表达,提高了TopoαmRNA水平.

关键词: 肝细胞癌, 多药耐药, 多药耐药逆转, 长春新碱, 柴胡

Abstract: The reversal effect and mechanisms of BCDC were evaluated by using Bel-7402 cell line with innate resistance in this study. The identification of new and less toxic reversal agent, BCDC, would open a new perspectives in cancer treatment. The results show that BCDC has an effect on MDR reversal of Bel-7402 cell line and a synergetic effect with VCR. The reversal mechanisms were due to suppress MDR1 transcription and P-gp expression, as a result, to leave TopoⅡα mRNA level, to increase intracellular VCR concentration of Bol-7402 cell, to enhance cell G2M phase arrest induced by VCR.

Key words: Hepatocellular carcinoma, Multidrug resistance(MDR), MDR reversal, Vincristine(VCR), Bupleurun Chinese DC

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