高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (10): 1829-1831.

• 研究简报 • 上一篇    下一篇

α1-酸性糖蛋白与药物相互作用的电喷雾离子阱质谱研究

陈勇, 祁春华, 韩凤梅   

  1. 湖北大学中药生物技术湖北省重点实验室, 武汉430062
  • 收稿日期:2005-01-06 出版日期:2005-10-10 发布日期:2005-10-10
  • 基金资助:

    湖北省杰出青年基金(批准号:2002AC004);中药生物技术湖北省重点实验室开放基金(批准号:040106)资助.

Studies on the Interaction of α1-Acid Glycoprotein and Drugs by Electrospray Ion Trap Mass Spectrometry

CHEN Yong, QI Chun-Hua, HAN Feng-Mei   

  1. Hubei Province Key Lab of Bio-Technology of Traditional Chinese Medicine,Hubei University,Wuhan 430062,China
  • Received:2005-01-06 Online:2005-10-10 Published:2005-10-10

关键词: 电喷雾离子阱质谱, &alpha, 1-酸性糖蛋白, 酮基布洛芬, 樟柳碱

Abstract: In this paper,the noncovalent interactions between α1-acid glycoprotein(AAG) and the drugs,such as ketoprofen(KP) and anisodine hydrobromide(AN) were studied by using electrospray ion trap mass spectrometry method.The results show that both of the drugs had a strong coalescent interaction with AAG,the(dissociation) constants(Kd) and the apparent stoichiometric ratio of noncovalent complexes AN-AAG were(1.75×10-3 mol/L) and 1∶7,and those for KP-AAG complex were 4.95×10-4 mol/L and 1∶4,respectively.According to the thermodynamic parameters of the noncoralent complexes,the main sorts of binding force were static-electricity gravitation for KP-AAG and Van der Waals force for AN-AAG,respectively. The experiment proves that ESI-MS technology as a new method for studying the drug-protein noncoralent binding can offer some advantages in sensitivity,speed,accuracy and directness particularly in the determination of the stoichiormetry of the complex compared to other well-established methods such as circular dichroism and fluorescence spectroscopy,etc.

Key words: Electrospray ion trap mass spectrometry(ESI-MS), α1-Acid glycoprotein, Ketoprofen, Anisodine hydrobromide

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