高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (10): 1803-1807.

• 研究论文 • 上一篇    下一篇

川芎嗪对脑缺血下大鼠纹状体区痕量氨基酸类神经递质的影响

党红梅, 马万云, 韩慧婉   

  1. 清华大学物理系原子分子纳米科学教育部重点实验室, 北京100084
  • 收稿日期:2005-01-18 出版日期:2005-10-10 发布日期:2005-10-10
  • 基金资助:

    国家自然科学基金(批准号:10274042,10474054)资助.

Influence of Ligustrazine on Trace Amino Acid Neurotransmitters in Striatum of Rats after Transient Focal Cerebral Ischemia by CZE-LIF

DANG Hong-Mei, MA Wan-Yun, HAN Hui-Wan   

  1. Key Laboratory for Atomic and Molecular Nanosciences of Education Ministry,Department of Physics,Tsinghua University,Beijing 100084,China
  • Received:2005-01-18 Online:2005-10-10 Published:2005-10-10

摘要: 采用脑内微透析技术结合激光诱导荧光检测的高效毛细管电泳研究了脑缺血下盐酸川芎嗪注射液对大鼠纹状体区氨基酸类神经递质含量的影响.在优化的分离条件下,各氨基酸在1×10-6-1×10-8mol/L的浓度范围内与荧光强度呈良好的线性关系,线性相关系数达到0.99以上,检测限低于1.6×10-9mol/L,适合于大鼠纹状体区微透析样品中的痕量氨基酸类神经递质的测量.实验结果表明,脑缺血时,使用盐酸川芎嗪注射液可显著降低细胞外兴奋性氨基酸神经递质谷氮酸(G lu)和天门冬氨酸(Asp)的浓度(P<0.05),同时可显著升高抑制性氨基酸神经递质γ-氨基丁酸(GABA)和甘氨酸(G ly)的浓度(P<0.05).这一结果将对阐明盐酸川芎嗪注射液治疗缺血性脑血管疾病的有关机理提供帮助.

关键词: 激光诱导荧光, 微透析, 氨基酸类神经递质, 脑缺血, 纹状体

Abstract: An effective coupling of microdialysis and capillary electrophoresis with laser induced fluorescence detection technique(CZE-LIF) was explored for the analysis of amino acid neurotransmitters in striatum of rat after transient focal cerebral ischemia in group treated with saline and group treated with hydrochloric acid(ligustrazine).The separation of amino acids was achieved within 30 min on an uncoated fused silica capillary(67 cm×50 μm i.d.) column with botate buffer(10 mmol/L,pH=10.35) and an applied voltage of(12.5 kV).With the current method,the fluorescence intensity has linear relationship with each amino acid concentration between 1×10-6-8-9

Key words: Laser-induced fluorescence, Microdialysis, Amino acid neurotramsmitter, Cerebral ischemia, Striatum

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