高等学校化学学报

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (1): 27.

• 研究论文 • 上一篇    下一篇

利用HPLC-ESI-MS/MS区分黄芩中黄酮C-苷异构体的研究

吴巍, 宋凤瑞, 刘志强, 刘淑莹   

  1. 中国科学院长春应用化学研究所新药研究实验室, 长春 130022
  • 收稿日期:2004-01-05 出版日期:2005-01-10 发布日期:2005-01-10
  • 通讯作者: 刘淑莹(1942年出生),女,博士,研究员,博士生导师,从事天然药物化学和有机质谱学研究.E-mail:mslab@ns.ciac.jl.cn E-mail:mslab@ns.ciac.jl.cn
  • 基金资助:

    国家重点科技攻关计划专题(批准号:99-909-02-09);吉林省科技发展计划重点项目(批准号:20030916-1)资助

Differentiation of the Flavonoid C-glycoside Isomers in Scutellaria baicalensis Georgi Using HPLC-ESI-MS/MS

WU Wei, SONG Feng-Rui, LIU Zhi-Qiang, LIU Shu-Ying   

  1. Laboratory of New Drug Research, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
  • Received:2004-01-05 Online:2005-01-10 Published:2005-01-10

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被引次数

35

摘要： 采用HPLC-ESI-MS/MS联用技术区分了黄芩中两种互为同分异构体的黄酮C-苷类化合物5,7-OH-6-C-阿拉伯糖-8-C-葡萄糖黄酮和5,7-OH-6-C-葡萄糖-8-C-阿拉伯糖黄酮.阐述了黄酮C-苷类化合物同分异构体的电喷雾串联质谱(ESI-MSn)的特征碎裂规律,证明了[M-H-60]-,[M-H-90]-及[M-H-120]-离子是其特征离子.实验结果表明,对于C-6位五碳糖取代和C-8位六碳糖取代的黄酮C-苷二级串联质谱产生的[M-H-60]-离子(0,35X),其丰度一般大于50%;而对于C-6位六碳糖取代和C-8位五碳糖取代的黄酮C-苷二级串联质谱产生的[M-H-60]-离子(0,35X),其丰度一般小于50%.据此可区分两种互为同分异构体的黄酮C-苷类化合物.

关键词: HPLC-ESI-MS/MS, 黄芩, 黄酮C-苷异构体

Abstract: HPLC-ESI-MS/MS is becoming one of the most important tools for the on-line identification of natural products in crude plant extracts. In this paper, a combined HPLC-ESI-MS/MS method was developed to character two flavonoid C-glycoside isomers in Scutellaria baicalensis Georgi. Using tandem mass spectrometry, useful fragments of the two flavonoid C-glycoside isomers were obtained. The experimental results demonstrate that cross-ring ions of [M-H-60]-, [M-H-90]-, [M-H-120]- are characteristic ions of flavonoid C-glycoside. By analyzing the differences of the retention time and the relative abundance of ions [M-H-60]-, the two isomers can be distinguished conveniently.

Key words: HPLC-ESI-MS/MS, Scutellaria baicalensis Georgi, Flavonoid C-glycoside isomers

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