高等学校化学学报 ›› 2012, Vol. 33 ›› Issue (07): 1450-1455.doi: 10.3969/j.issn.0251-0790.2012.07.015

• 分析化学 • 上一篇    下一篇

紫外光谱法结合多元曲线分辨研究黄芩苷的转化规律

郑辛甜, 瞿海斌   

  1. 浙江大学药物信息学研究所, 杭州 310058
  • 收稿日期:2011-06-30 出版日期:2012-07-10 发布日期:2012-07-10
  • 通讯作者: 瞿海斌, 男, 博士, 教授, 博士生导师, 主要从事中药质量控制和过程分析研究. E-mail: quhb@zju.edu.cn E-mail:quhb@zju.edu.cn
  • 基金资助:

    "重大新药创制"国家科技重大专项(批准号: 2009ZX09313-036)资助.

Characterization of Transformation Pattern of Baicalin Using UV Spectrometric Analysis and Multivariate Curve Resolution

ZHENG Xin-Tian, QU Hai-Bin   

  1. Pharmaceutical Informatics Institute, Zhejiang University, Hangzhou 310058, China
  • Received:2011-06-30 Online:2012-07-10 Published:2012-07-10

摘要: 采用紫外光谱法采集不同工艺条件下黄芩苷转化过程的光谱数据, 结合多元曲线分辨-交替最小二乘法(MCR-ALS)处理获得的数据, 得到黄芩苷转化反应过程中各组分的光谱图和浓度随时间变化曲线. 结果表明, 黄芩苷转化过程符合两步连续一级反应模型; 根据不同时间点采集的多级质谱和飞行时间质谱信息, 推断了黄芩苷转化产物和可能的转化途径, 转化产物主要包括黄芩苷的同分异构体、 黄芩素和黄芩素二聚体. 对黄芩苷转化规律的研究有助于黄芩制剂的质量控制.

关键词: 黄芩苷, 紫外光谱, 多元曲线分辨-交替最小二乘法

Abstract: Investigation of the transformation pattern of bacalin contributes to the quality control of scutellaria preparations. In this work, UV spectrometric method was used to record the UV spectra during transformation process of baicalin under different conditions. Multivariate curve resolution was utilized to analysis the spectra for resolving concentration and spectra profiles of the reactant species. The transformation kinetic profile of baicalin was evaluated as consecutive two-step one-order reaction. Transformation products were deduced by high performance liquid chromatography-tandem multistage mass spectrometric and time-of-flight mass spectrometric analysis of samples collected at different times during reaction process. The transformation products of baicalin included isomerides of baicalin, baicalein and dimmer of baicalein. The possible transformation pathway of baicalin was also inferred.

Key words: Baicalin, UV spectra, Multivariate curve resolution-alternating least square

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