高等学校化学学报

高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (8): 1454.

• 研究论文 • 上一篇    下一篇

IgA肾病免疫吸附剂的研究(Ⅲ)

褚佳强, 张卫华, 俞耀庭, 朱伯儒, 陈长治   

  1. 南开大学教育部活性材料研究重点实验室, 天津300071
  • 收稿日期:2003-07-21 出版日期:2004-08-24 发布日期:2004-08-24
  • 基金资助:

    国家重点基础研究与发展基金(“九七三”计划)(批准号:1999064707);天津大学南开大学共建教育部基金资助

In Vitro Studies of the Immunoadsorbent for Removal of IgA in IgA-nephropathy (Ⅲ)

CHU Jia-Qiang, ZHANG Wei-Hua, YU Yao-Ting, ZHU Bo-Ru, CHEN Chang-Zhi   

  1. State Key Lab of Bioactive Materials Research, Nankai University, Tianjin 300071, China
  • Received:2003-07-21 Online:2004-08-24 Published:2004-08-24

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摘要： 在碱性条件下,用环氧氯丙烷对琼脂糖载体进行活化,并通过戊二醛法引入手臂,偶联羊抗人IgA的抗体,清除IgA肾病病人体内高含量的IgA分子,从而达到治疗病症的目的.通过体外实验发现,新研制的免疫吸附剂最高吸附率可以达到70%左右.

关键词: IgA肾病, 琼脂糖, 免疫吸附剂

Abstract: IgA nephropathy is a kidney disorder caused by deposits of IgA inside the glomeruli of the kidney. This chronic disease may progress over a period of 10 to 20 years and usually cannot be cured effectively. Immunoadsorbent can remove the excess IgA by hemoperfusion. We prepared the adsorbent by reacting agarose with epoxy chloropropane to introduce the active epoxy group. Then it was reacted with ammonium hydroxide and glutaraldehyde to introduce a spacer and sheep anti-human IgA antibody was linked to the spacer as a ligand. In vitro experiments showed that the adsorbent thus prepared could remove excess IgA in plasma effectively and the adsorption capacity reached 74%.

Key words: IgA-nephropathy, Agarose, Immnoadsorbent

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