氮酮对鼠角质层角蛋白作用的光谱证据

高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (7): 1273.

氮酮对鼠角质层角蛋白作用的光谱证据

周雪琴^1,2, 许晶¹, 赵晓东¹, 张志慧¹, 姚康德¹, 刘东志²

1. 天津大学高分子材料研究所;
2. 天津大学化工学院, 天津 300072

收稿日期:2003-06-03 出版日期:2004-07-24 发布日期:2004-07-24
通讯作者: 周雪琴(1974年出生),女,博士,副教授,从事功能材料与药物制剂研究.E-mail:whoopee@public.tpt.tj.cn E-mail:whoopee@public.tpt.tj.cn
基金资助:
国家重点基础研究发展规划项目(批准号:G199954305)资助

Spectroscopic Evidence of Azone Effect on the Keratin in Mouse Stratum Corneum

ZHOU Xue-Qin^1,2, XU Jing¹, ZHAO Xiao-Dong¹, ZHANG Zhi-Hui¹, YAO Kang-De¹, LIU Dong-Zhi²

1. Research Institute of Polymeric Materials;
2. School of Chemical Engineering, Tianjin Unviersity, Tianjin 300072, China

Received:2003-06-03 Online:2004-07-24 Published:2004-07-24

摘要/Abstract

摘要： 应用13CNMR和ATR-FTIR为测试手段,探讨角蛋白的分子运动自由度与堆积结构,以及氮酮对鼠角质层角蛋白的作用.经氮酮处理后,角蛋白主侧链碳的自旋晶格弛豫时间t1从7.9,8.2和2.1s分别减小到5.4,3.6和1.6s,表明氮酮作用加快了角蛋白所有碳的运动,获得了氮酮对鼠角质层角蛋白作用的确凿证据.同时,ATR-FTIR结果显示,经氮酮处理后,角蛋白的酰胺吸收峰带的峰位从1544.7cm^-1向低波数位移到1541.4cm^-1.结果表明氮酮可使部分角蛋白从α-螺旋型结构向β-折叠型结构和无规则卷曲型结构转变,导致角蛋白的堆积结构疏松,增大了角蛋白碳运动的自由度

关键词: 氮酮, 角蛋白, ¹³CNMR, 自旋晶格弛豫时间t₁

Abstract: Chemical enhancers are widely employed to overcome the skin barrier in transdermal drug delivery systems. The penetration mechanism of chemical enhancers is the key point to develop a new transdermal drug delivery system. Azone is a common chemical enhancer and could make the stratum corneum(SC) lipid fluidization. However the protein also contributes to the skin barrier. Transport of large molecules was blocked by the keratin matrix even with an aqueous lipid pathway under a high-voltage pulsing. In this paper the effects of azone on the keratin in the mouse SC were investigated by determination of the molecular mobility and secondary structure of keratin. ¹³C NMR spectroscopy recorded a decreased spin-lattice relaxation time of keratin(t₁) for the carbon in the main and graft chain when treated with azone. ATR-FTIR spectra showed that amide Ⅱ absorption peak of keratin shifted from 1 544.7 to 1 541.4 cm^-1 after the treatment with azone. It was concluded that azone could change the keratin conformation structure partially from α-helix to β-sheet or random coil, leading to a loose keratin structure, therefore reduced the drug transdermal delivery barrier.

Key words: Azone, Keratin, ¹³C NMR, Spin-lattice relaxation time t₁

中图分类号:

Q95-3

TrendMD:

周雪琴, 许晶, 赵晓东, 张志慧, 姚康德, 刘东志. 氮酮对鼠角质层角蛋白作用的光谱证据. 高等学校化学学报, 2004, 25(7): 1273.

ZHOU Xue-Qin, XU Jing, ZHAO Xiao-Dong, ZHANG Zhi-Hui, YAO Kang-De, LIU Dong-Zhi . Spectroscopic Evidence of Azone Effect on the Keratin in Mouse Stratum Corneum. Chem. J. Chinese Universities, 2004, 25(7): 1273.

参考文献

[1] ZHEN Jun-Min(郑俊民).Transdermal Drug Delivery Syst em(经皮给药新剂型)[M],Beijin g:People Sanit at ion Pres s,1999: 36—178 [2] Naik A.,Kal ia Y.H.,Guy R.H..Pharma.Sci.Technol.Today[J],2000,3(9):318—326 [3] Praus nit z M.R..Adv.Drug Deliver y Rev.[J],1999,35:61—76 [4] DIN G Ping-Tian(丁平田),HA O Jin-Song(赫劲松),ZHE N Jun-Min(郑俊民).Act a Bioph ysica Si nica(生物物理学报)[J], 2000,16(1):48—52 [5] HA O Jin-Song(赫劲松),ZHEN Jun-Min(郑俊民),ZHANG Ru-Hu a(张汝华)et al..Act a Bioph ysica Sin ica(生物物理学报) [J],1995,11(4):587—590 [6] Q I Guo-Rong(戚国荣),YANG Yun-Song(杨云松),YANG Shi-Lin(杨士林)et al..Chinese Journal of App lied Chemist r 化学)[J],1997,14(4):25—28 [7] Lin Shan-Yan g,Duan Kw o-Jen,Lin Ts ung-Chien.Spect roch imica Act a,Part A[J],1996,52:1 671—1 678 [8] Angel is A.D.,Capit ani D.,Cres cenzi V..Macromolecules[J],1998,31:1 595—1 602 [9] Mot ohiro M.,At s uki H.,Hidekazu M.et al..Macromol ecu les[J],2002,35:2 595—2 601 [10] G A O Han-Bing(高汉宾),ZHEN Yao-Hua(郑耀华).Concis e Handb ook of N M R Spect roscopy(简明核磁共振手册)Wuh an:Hubei Science&Tech nology Press,1989:9—13 [11] St een R.G.,Schroeder J..Magn.Reson.Im agin g[J],2003,21(1):9—15 [12] Bart on S.J.,Howe F.A.,Toml ins A.M.et al..Mag net ic Res on ance Materials in Bi ol ogy,Phys ics and Medicine[J], 8(2):121—128 [13] SHEN Qi-Fen g(沈其丰).13C N M R Spect ra(核磁共振碳谱)[M],Bei jing:Peking Un iversit y Pres s,1988:176—211 [14] ZHA N G Kai-Ming(张开明),WANG Gang(王　刚),Y IN Guo-Hua(尹国华).Current Skin Science Theory&Pract ice(最科学理论与实践)[M],Beijing:Chinese Medical Science&Technology Press,2001:1—36 [15] HE You-Jie(何有节),ZHA N G Mai-Hua(张迈华),LIU Qi-Ze(刘其则)et al..Leath er Science and Engineerin g(皮革科程)[J],1990,(2):5—10 [16] Xue G.,Ji G.,Yan H.et al..Macromolecules[J],1998,31:7 706—7 711 [17] Guo M..Macromol ecules[J],1997,30:1 234—1 235 [18] Perez E.,Vanderhart D.L.,Cris t B.et al..Macromol ecu les[J],1987,20:78—87