高等学校化学学报

高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (7): 1268.

• 研究论文 • 上一篇    下一篇

aFGF拮抗剂对3T3细胞蛋白质组影响的研究

李小娟1,2, 付玉芹2, 高贵1, 崔银秋2, 周慧2   

  1. 1. 吉林大学分子酶学工程教育部重点实验室;
    2. 吉林大学生命科学学院生物大分子实验室, 长春 130023
  • 收稿日期:2003-07-07 出版日期:2004-07-24 发布日期:2004-07-24
  • 通讯作者: 周慧(1954年出生),女,博士,教授,博士生导师,从事生物化学与分子生物学研究.E-mail:zhouhui@mail.jlu.edu.cn E-mail:zhouhui@mail.jlu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:30070892)资助

Research on the Influence of aFGF Antagonist on 3T3 Cell Protein Profiles

LI Xiao-Juan1,2, FU Yu-Qin2, GAO Gui1, CUI Yin-Qiu2, ZHOU Hui2   

  1. 1. Key Lab for Molecular Enzymology and Engineering, Ministry of Education;
    2. Macromolecular Lab, College of Life Science, Jilin University, Changchun 130023, China
  • Received:2003-07-07 Online:2004-07-24 Published:2004-07-24

PDF (PC)

被引次数

3

摘要： 六肽P2(VYMSPF)是我室从噬菌体展示肽库中筛选出来的aFGF拮抗剂,为研究其对aFGF信号传导机制的影响,对处于4种不同生理条件下的NIH3T3细胞(正常的细胞、加P2刺激的细胞、加aFGF+肝素刺激的细胞、加aFGF+肝素+P2刺激的细胞)的全细胞裂解液进行双向电泳分离及软件分析.对后2种细胞的蛋白质图谱中表达差异的5个蛋白质点进行质谱分析和数据库检索.鉴定出3种表达下调的蛋白质,其中鸟苷酸结合蛋白α-11亚单位和1C-型核因子分别参与细胞内aFGF信号传导以及转录调控.这些差异点的变化为进一步研究P2对aFGF信号传导途径的抑制作用提供了实验基础和线索

关键词: 成纤维细胞生长因子, 六肽, 双向电泳

Abstract: The peptide P2(VYMSPF) selected from the phage display peptide library is an antagonist of aFGF, but its mechanism of influence on aFGF signal transduction is not clear. We used the two-dimensional electrophoresis and mass spectrometry technique to study the NIH3T3 cell cultured in the blank IMDM medium and the IMDM mediums containing peptide P2, aFGF+heparin, aFGF+heparin+peptide P2 respectively. We selected five protein spots with altered expression in the latter two samples to perform mass spectrometry analysis and database search. Among the identified proteins, expression of three proteins was evidently decreased, including the guanine nucleotide-binding protein, alpha-11 subunit involved in the interior cellular signal transduction and nuclear factor 1C-type involved in the transcription regulation. In summary, these proteins provided the experimental base and clue for further study of the inhibition effect of P2 on aFGF signal transduction.

Key words: Fibroblast growth factor, Six peptide, Two-dimensional electrophoresis

中图分类号: 

TrendMD: