高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (3): 445.

• 研究快报 • 上一篇    下一篇

Fe3O4/葡聚糖/抗体磁性纳米生物探针的制备和层析检测

洪霞1, 郭薇1, 李军1, 刘艳梅1, 杨文胜1, 白玉白1, 李铁津1, 袁航2, 马岚2   

  1. 1. 吉林大学化学学院, 长春 130023;
    2. 云南大学单克隆抗体工程技术研究中心, 昆明 650091
  • 收稿日期:2003-09-16 出版日期:2004-03-24 发布日期:2004-03-24
  • 通讯作者: 白玉白(1941年出生),男,教授,博士生导师,从事凝聚态光物理与光化学研究.E-mail;yubai@jlu.edu.cn E-mail:yubai@jlu.edu.cn
  • 基金资助:

    国家自然基金项目(批准号;20073016;60271020);国家“973”计划项目(批准号;G1999064504);“863”计划项目(批准号;2002AA302203)资助

Preparation of Nano-scaled Magnetic Biological Probes of Fe3O4/Dextran/Antibody and Chromatographic Assay

HONG Xia1, GUO Wei1, LI Jun1, LIU Yan-Mei1, YANG Wen-Sheng1, BAI Yubai1, LI Tie-Jin1, YUAN Hang2, MA Lan2   

  1. 1. College of Chemistry, Jilin University, Changchun 130023, China;
    2. Monoclonal Antibody Technology Center, Yunnan University, Kunming 650091, China
  • Received:2003-09-16 Online:2004-03-24 Published:2004-03-24

关键词: 磁性生物探针, 抗原-抗体识别, BSA封闭, 层析检测

Abstract: Dextran coated magnetic nanocomposites were prepared. A periodate oxidation method was used to further activate the magnetic dextran, forming magnetic polyaldehyde dextran, which could be conjugated to biomolecules, antibodes. The magnetic labeled nano scaled bioconjugates could be well blocked with BSA and exhibit high activity by ELISA and immunochromatographic strip assay.

Key words: Magnetic biological probes, Antigen antibody recognition, BSA Blocking, Chromatographic assay

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