高等学校化学学报

高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (3): 425.

• 研究论文 • 上一篇    下一篇

基于功能化溶胶-凝胶膜的乳腺癌抗原免疫传感器

梁汝萍1,2, 邱建丁1,2, 邹小勇1, 蔡沛祥1   

  1. 1. 中山大学化学与化学工程学院, 广州 510275;
    2. 萍乡高等专科学校化工系, 萍乡 337055
  • 收稿日期:2003-01-20 出版日期:2004-03-24 发布日期:2004-03-24
  • 通讯作者: 蔡沛祥(1940年出生),男,教授,博士生导师,从事电分析化学研究.E-mail;caipx@21cn.com E-mail:caipx@21cn.com
  • 基金资助:

    广东省自然科学基金(批准号;011105;031577)资助

Breast Cancer Antigen Immunosensor Based on the Functional Sol-gel Film

LIANG Ru-Ping1,2, QIU Jian-Ding1,2, ZOU Xiao-Yong1, CAI Pei-Xiang1   

  1. 1. School of Chemistry and Chemical Engineering, Zhongshan University, Guangzhou 510275, China;
    2. Department of Chemical Engineering, Pingxiang College, Pingxiang 337055, China
  • Received:2003-01-20 Online:2004-03-24 Published:2004-03-24

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被引次数

19

摘要： 结合溶胶-凝胶(sol-gel)和交联技术,将乳腺癌抗体固定在铂盘电极表面的氨基化sol-gel功能膜上,制备出用于检测乳腺癌抗原(CA15-3)的免疫传感器.用红外光谱验证了该传感器功能膜的形成过程和组成结构,采用循环伏安法对传感器逐层修饰过程进行了表征,并对传感器功能膜的作用机理进行了探讨.该法检测CA15-3的线性范围为8～240U/mL,线性回归方程为ΔE=75.75lgc-56.36,r=0.998.结果表明,该方法很好地保持了被固定抗体的活性,增强了传感器的稳定性,所制备的传感器于4℃干态保存30d,其响应信号基本不变.且该传感器响应迅速、灵敏度高、选择性好,血清中常见抗原不干扰测定.

关键词: 溶胶-凝胶, 交联技术, 乳腺癌, 免疫传感器

Abstract: A new type of non labeled immunosensor for the determination of breast cancer antigen(CA15 3) was made by combining sol gel with cross link techniques and utilizing glutaraldehyde(GA) to link CA15 3 antibody(Ab) on the functional sol gel film, so the sol gel/GA/Ab layer was immobilized on the surface of platinum disk electrode. Infrared spectrum(IR) and cyclic voltammetry(CV) were employed to investigate the structure and the electrochemical characteristics of the immunosensor, respectively. The linearity of CA15 3 in the range of 8—240 U/mL with a detection limit of 5 U/mL and the correlation coefficient of 0 998 are obtained. The experimental results show that the activity of the immobilized CA15 3 antibody is maintained better by this method, and the stability of the immunosensor is improved. The dependences of the potential response on pH, incubation time, sensitivity and reproducibility were studied, and the stability of the sensor was also evaluated. The immunosensor was found to be stable for about 30 days when stored in a dry state at 4 ℃. Satisfactory determination results of CA15 3 in serum samples were obtained by this method.

Key words: Sol gel, Cross link technique, Breast cancer, Immunosensor

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