高等学校化学学报

高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (1): 44.

• 研究论文 • 上一篇    下一篇

压电胰岛素-C肽微阵列免疫传感器研究

张波1, 府伟灵1, 毛琼国1, 张雪1, 汤万里2, 俞凡3   

  1. 1. 第三军医大学西南医院检验科, 重庆 400038;
    2. 中国嘉陵集团生物工程办公室, 重庆 400032;
    3. 信息产业部电子第26研究所, 重庆 400060
  • 收稿日期:2002-09-06 出版日期:2004-01-24 发布日期:2004-01-24
  • 通讯作者: 府伟灵(1955年出生),男,教授,博士生导师,主要从事病原微生物快速诊断和压电生物传感器研究.E-mail:weilingfu@yahoo.com E-mail:weilingfu@yahoo.com
  • 基金资助:

    国家科技部“九五”科学仪器攻关项目(批准号:96-A23-04-04);国家高技术计划生物技术领域青年基金;国家自然科学基金(批准号:39870831);解放军“九五”;“十五”课题(批准号:98M064,01MA180,01LO065)资助

Studies on Piezoelectric Insulin-C Peptide Micro-array Immunosensor

ZHANG Bo1, FU Wei-Ling1, MAO Qiong-Guo1, ZHANG Xue1, TANG Wan-Li2, YU Fan3   

  1. 1. Department of Laboratory, Southwest Hospital, Third Military Medical University, Chongqing 400038, China;
    2. Department of Bioengineering, China Jialing Group, Chongqing 400032, China;
    3. The 26th Research Institute of Electronics, Ministry of Information Industry, Chongqing 400060, China
  • Received:2002-09-06 Online:2004-01-24 Published:2004-01-24

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被引次数

15

摘要： 以AT切型、基频10MHz的镀金膜石英晶体作为换能器,将抗人胰岛素和C肽单克隆抗体固定在石英晶体电极表面,用2×5检测池固定夹具构建一种新型压电胰岛素-C肽微阵列免疫传感器.研究了抗体固定方法、抗体工作浓度、固定量、一致性以及传感器的响应参数如检测温度、时间和特异性等的影响.该微阵列传感器在胰岛素浓度为2.5～160.0mIU/L、C肽浓度为0.375～12.0ng/mL范围内响应特性良好,压电晶体频率偏移值与胰岛素和C肽浓度呈良好的线性关系.将此微阵列传感器用于人血清标本的测定,结果与放射免疫法符合(r为0.92和0.94).此微阵列传感器具有灵敏度高、特异性好,低密度阵列结构,检测通量较高,不需标记,操作简单、能实时在线检测和重复使用等优点,能用于临床实验诊断,具有临床推广应用价值.

关键词: 压电免疫传感器, 微阵列, 胰岛素, C肽

Abstract: A new 2×5 type of piezoelectric insulin-C peptide micro-array immunosensor was developed for the determination of insulin and C peptide. The energy converters were 10 MHz AT-cut quartz crystals with gold-coated electrodes. The monoclonal antibodies of insulin and C peptide were immobilized onto the surfaces of crystals gold electrodes respectively, then piezoelectric insulin-C peptide micro-array immunosensor was constructed by 2×5 type of immobilization clamping apparatus. The effects of methods, work concentrations, the amount and coherence of immobilization of antibody and the response parameters of the micro-array immunosensor such as temperature, time-cost and specificity etc. were investigated. Under the optimized conditions, the experimental results showed piezoelectric immunosensor had good response to insulin and C peptide whose frequency shifts were linearly dependent on insulin concentration in the range of 2.5—160.0 mIU/L and C peptide concentration in the range of 0.375—12.0 ng/mL. The piezoelectric micro-array immunosensor were used to detect insulin and C peptide in serum, the analytical results given by this approach were in satisfactory agreement with those given by radiometric immunoassay(P>0 05), with correlation coefficients 0 92 and 0 94. The piezoelectric micro-array immunosensor for the determination of insulin and C peptide is of high sensitivity, high specificity, low density micro-array structure, high analysis speed, unnecessary labelling, simple operation, real-time detection and repeated use, etc.. It is suitable for detecting insulin and C peptide of serum in clinical laboratory.

Key words: Piezoelectric immunosensor, Micro-array, Insulin, C peptide

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