高等学校化学学报

高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (9): 1621.

• 研究简报 • 上一篇    下一篇

DNA共价结合在化学修饰云母片上的AFM研究

祭美菊, 侯鹏, 沈佳尧, 李松, 陆祖宏, 何农跃   

  1. 东南大学教育部分子与生物分子电子学开放实验室, 南京 210096
  • 收稿日期:2002-08-14 出版日期:2003-09-24 发布日期:2003-09-24
  • 通讯作者: 何农跃(1958年出生),男,教授,博士生导师,从事生物芯片和纳米材料方面的研究.E-mail:nyhe@aeu.edu,cn E-mail:nyhe@aeu.edu.cn.;zhlu@.seu.edu.cn.
  • 基金资助:

    国家重大基础研究项目(批准号:G1998051204);国家自然科学基金(批准号:60071001)

AFM Studies on DNA Covalently Immobilized on Mica Modified by APTES-GA

JI Mei-Ju, HOU Peng, SHEN Jia-Yao, LI Song, LU Zu-Hong, HE Nong-Yue   

  1. Key Laboratory for Molecular and Bio-molecular Electronics of Ministry of Education, Nanjing 210096, China
  • Received:2002-08-14 Online:2003-09-24 Published:2003-09-24

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被引次数

14

摘要： 原子力显微镜(AFM)自1986年发明以来,已经成为生物学研究领域中的一个有效工具,尤其在核酸及其它生物大分子结构方面的应用已成为普遍关注的热点.原子力显微镜要求基底达到原子级平整,硅片和玻璃表面的起伏很大,因而原子级平整的云母具有重要的价值.

关键词: 脱氧核糖核酸(DNA), 原子力显微镜(AFM), 共价偶联

Abstract: The immobilization of DNA on self-assembled layers of 3-aminopropyltrimethoxysilane (APTES) and glutaraldehyde(GA) on mica wafers by chemical coupling has been studied by using atomic force microscopy (AFM). The DNAused for our investigation is amplified by PCR and primers were labeled by -NH2at their 5' terminal. The surfaces were analyzed with X-ray photoelectron spectroscopy (XPS) and AFM. The results from XPS and AFM show that APTES-GA modified mica can be formed and the flatness of mica is adapted to AFM images. We found that DNA can be readily immobilized on mica. The covalent coupling between the aldehyde-terminated membrane and the both H2N-groups in double strands DNA results in the immobilization of the DNA straightly.

Key words: DNA, Atomic force microscopy(AFM), Covalent coupling

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