高等学校化学学报

高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (7): 1175.

• 论文 • 上一篇    下一篇

水溶液微悬浮聚合法制备酸性药物吲哚美辛分子印迹微球及其色谱表征

赖家平, 卢春阳, 何锡文   

  1. 南开大学化学系, 吸附分离功能高分子材料国家重点实验室, 天津 300071
  • 收稿日期:2002-06-21 出版日期:2003-07-24 发布日期:2003-07-24
  • 通讯作者: 何锡文(1939年出生),男,教授,博士生导师,主要从事分子印迹和分子识别研究.E-mail:xiwenhe@nankai.edu.cn E-mail:xiwenhe@nankai.edu.cn
  • 基金资助:

    吸附分离功能高分子材料国家重点实验室开放基金(南开大学);国家自然科学基金(批准号:20175009);教育部博士学科点基金(批准号:20020055002)资助

Preparation of Molecularly Imprinted Microspheres Against Acidic Indometacin and Its Chromatographic Characterization by Aqueous Micro-suspension Polymerization

LAI Jia-Ping, LU Chun-Yang, HE Xi-Wen   

  1. State Key Laboratory of Functional Polymer Materials for Adsorption and Separation, Department of Chemistry, Nankai University, Tianjin 300071, China
  • Received:2002-06-21 Online:2003-07-24 Published:2003-07-24

PDF (PC)

被引次数

189

摘要： 以带有羧基的酸性药物吲哚美辛为模板分子、碱性的4-乙烯基吡啶为功能单体,采用水溶液微悬浮聚合法制备了用于色谱分离的微米级分子印迹微球.详细讨论了流动相中缓冲溶液的pH值对吲哚美辛在MIMs柱上的容量因子(k′)、分离因子(α)和印迹因子(β)的影响.通过MIMs柱对吲哚美辛和4-氨基吡啶(4-AP)的保留行为的比较,证明以4-乙烯基吡啶为功能单体制得的MIMs对吲哚美辛的识别作用,主要靠吡啶环上氮原子与吲哚美辛羧基之间的离子键相互作用,以及吡啶环与模板分子之间的π-π相互作用.

关键词: 分子印迹微球(MIMs), 分子印迹聚合物(MIPs), 水溶液微悬浮聚合法, 吲哚美辛

Abstract: The molecularly imprinted microspheres(MIMs) for high performance liquid chromatography were synthesized by aqueous micro-suspension polymerization. The acidic indometacin, one of the antidepressants, was used as the template and basic 4-vinylpyridine was used as functional monomer. The influences of pH of mobile phase on the capacity factor(k′), separation factor(α) and selection factor(β) of indometacin were investigated in detail. All the results showed that the MIMs exhibited specific recognition and affinity to indometacin only in pH<4.0 eluent; and the specific recognition of MIMs was lost gradually in pH>4.0 eluent. By comparing the retention behavior of indometacin and 4-aminopyridine (4-AP) on the MIMs column, it proved that the recognition of MIMs to indomeatcin synthesized by 4-vinylpyridine as a cross-linker mainly relied on the ion-pair interaction between the nitrogen atom on the pyridine ring and the carboxyl on the indometacin, and the π-π interaction between the pyridine ring and the template molecule. This was just contrary to that MIMs synthesized by methacrylic acid as the cross-linker.

Key words: Molecularly imprinted microspheres(MIMs), Molecularly imprinted polymers(MIPs), Aqueous suspension polymerization, Indometacin

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