高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (4): 588.

• 论文 • 上一篇    下一篇

具有甲状腺素脱碘酶活性的抗体酶的化学修饰

孙立伟, 刘立岩, 赵大庆   

  1. 中国科学院长春应用化学研究所稀土化学与物理开放实验室, 长春 130022
  • 收稿日期:2002-04-16 出版日期:2003-04-24 发布日期:2003-04-24
  • 通讯作者: 赵大庆(1963年出生),男,博士,研究员,博士生导师,主要从事生物无机化学研究.E-mail:dqzhao@ciac.jl.cn E-mail:dqzhao@ciac.jl.cn
  • 基金资助:

    国家自然科学基金重大项目(批准号:29890280)

Chemical Modification of Abzyme with Deiodinase Activity

SUN Li Wei, LIU Li Yan, ZHAO Da Qing   

  1. Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
  • Received:2002-04-16 Online:2003-04-24 Published:2003-04-24

关键词: 必需基团, 化学修饰, 抗体酶

Abstract: The abzyme with deiodinase activity was modified with diethyl pyrocarbonate(DEPC) and carbodiimide(EDC) respectively. The modified abzymes were dialyzed and their activities were compared with that of the native abzyme. The results indicate that the histidine residue is one of the essential groups in the abzyme, and the modification causes the catalytic activity a loss of 70%. The modification of the residues containing carboxyl group with EDC leads to a loss of 50% activity, indicating that acid amino residues play important roles in the catalytic reaction.

Key words: Essential groups, Chemical modification, Abzyme

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