高等学校化学学报

高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (10): 1775.

• 论文 • 上一篇    下一篇

提高芯片毛细管电泳系统检测信噪比的哈达玛变换法

张涛1, 方群2, 方肇伦1,2   

  1. 1. 东北大学分析科学研究中心, 沈阳 110006;
    2. 浙江大学化学系, 微分析系统研究所, 杭州 310028
  • 收稿日期:2002-11-26 出版日期:2003-10-24 发布日期:2003-10-24
  • 通讯作者: 方肇伦(1934年出生),男,教授,博士生导师,中国科学院院士,从事微流控系统研究.E-mail:fangzl@mail.hz.z.jcn E-mail:fangzl@mail.hz.z.jcn
  • 基金资助:

    国家自然科学基金(批准号:20299030)资助

Enhancement of Signal-to-Noise Ratio in Chip-based Capillary Electrophoresis Systems by a Hadamard Transform Approach

ZHANG Tao1, FANG Qun2, FANG Zhao-Lun1,2   

  1. 1. Research Center for Analytical Sciences, Northeastern University, Shenyang 110006, China;
    2. Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310028, China
  • Received:2002-11-26 Online:2003-10-24 Published:2003-10-24

PDF (PC)

被引次数

12

摘要： 采用门控进样,在简单的十字通道微流控玻璃芯片上实现了假随机多次进样,研究了利用哈达玛变换提高微流控毛细管电泳分析系统信噪比的方法.在实验中,以7阶127步假随机二进制序列作为进样模板,将缓冲液和Cy5衍生后的氨基酸试样交替注入到分离通道中,检测到的电泳信号经过哈达玛反变换还原使信噪比提高5倍(理论上5.6倍)的电泳谱,各组分的出峰时间、峰高和峰形均完全还原,毛细管电泳分离的采样频率不受影响.

关键词: 微流控分析系统, 芯片毛细管电泳, 哈达玛变换, 信噪比, 数字信号处理

Abstract: The first demonstration of Hadamard transform capillary electrophoresis(CE) is reported for enhancing the signal-to-noise ratios(S/N) on a microfluidic chip using a simple electrokinetically gated injection approach in this paper.Sample or running buffer was alternatively injected into the separation channel according to a 7-bit, 127-step pseudorandom binary sequence(PRBS).The electrophoregram rebuilt from detector signal with inverse Hadamard transform improved the S/Nratio by a factor of 5(5.6 in theory), compared to conventional single injection electrophoregram, almost without affecting the sample throughput.

Key words: Microfluidic control analysis system, Chip-based capillary electrophoresis, Hadamard transform, Signal-to-noise ratio, Digital signal processing

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