高等学校化学学报

高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (6): 1050.

• 研究简报 • 上一篇    下一篇

微柱液相色谱-电化学法检测鼠纹状体单胺类物质的含量

王磊, 韩慧婉, 余晓, 赵睿, 刘国诠   

  1. 中国科学院化学研究所, 分子科学中心, 北京 100080
  • 收稿日期:2001-07-27 出版日期:2002-06-24 发布日期:2002-06-24
  • 通讯作者: 韩慧婉(1943年出生),女,研究员,从事微透析和生化分析研究.
  • 基金资助:

    国家自然科学重点基金(批准号:20035010);中国科学院重大基础研究项目(批准号:KJ951-A1-507)资助

Detection of Monoamines in Rat Striatum with Microbore HPLC-ECD

WANG Lei, HAN Hui-Wan, YU Xiao, ZHAO Rui, LIU Guo-Quan   

  1. Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China
  • Received:2001-07-27 Online:2002-06-24 Published:2002-06-24

PDF (PC)

被引次数

20

关键词: 微柱液相色谱, 单胺, 微透析, 纹状体

Abstract: Using microbore HPLCand electrochemical detection, Amicrodialysis HPLCassay system was built. After investigating the linear range, detection limit and reproducibility, this method was used to measure the concentrations of DAand monoamine metabolites in striatal microdialysate with 5 min temporal resolution. The detection limit is less than 5 nmol/L. According to the statistical analysis, the result is stable and accurate. It is very helpful to the physiological, pathological and pharmacological research.

Key words: Microbore HPLC, Monoamines, Microdialysis, Striatum

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