高等学校化学学报

高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (10): 1887.

• 研究论文 • 上一篇    下一篇

重症肌无力免疫吸附剂的制备及性能研究

颜伟荣, 俞耀庭, 杨丽, 张卫华, 缪鹏冲   

  1. 南开大学生物活性材料教育部重点实验室, 天津 300071
  • 收稿日期:2001-07-17 出版日期:2002-10-24 发布日期:2002-10-24
  • 通讯作者: 俞耀庭(1926年出生),男,教授,博士生导师,从事医用高分子材料化学及生物学研究.
  • 基金资助:

    973重大基础项目“生物医用材料基本科学问题的研究”(批准号:G1999064707)资助

Studies on New Immunoadsorbent for Myasthenia Gravis

YAN Wei-Rong, YU Yao-Ting, YANG Li, ZHANG Wei-Hua, MIAO Peng-Chong   

  1. The Key Laboratory of Bioactive Material of Minstry of Education, Nankai University, Tianjin 300071, China
  • Received:2001-07-17 Online:2002-10-24 Published:2002-10-24

PDF (PC)

被引次数

26

摘要： 重症肌无力患者体内致病毒素是乙酰胆碱的受体抗体.通过对载体材料的优化及对配基的筛选发现,使用质量分数为6%的纤维素球形载体,以环氧氯丙烷法活化并固定上L-色氨酸作为配基制备的血液灌流材料在静态吸附实验(1g吸附剂加入3mL病人血清,室温,3h)中可使抗体水平下降25%以上,吸附量达3.669×10-3nmol/g,选择性较高,具有应用前景.

关键词: 重症肌无力, 乙酰胆碱受体抗体, 免疫吸附剂

Abstract: Myasthenia Gravis(MG) is an serious nerval specific autoimmune disease. The pathogenic factor of the disease is the anti-AChR antibodies in the blood of patients which react against the acetylcholine receptor(AChR). Literature reported that the AChR ab could be adsorbed by adsorbent linked with special amino acids or proteins. In order to remove AChr ab from blood, we studied the selectivity and adsorption capacity of various ligands and matrixes. Experimental results showed that immunoadsorbent prepared by coupling L-tryptophan to epichlorohydrin-activated cellulose bead(6%) gave the best result. Adsorbent of 1.0 g in 3.0 mL of serum could remove 25% of antibody from the serum, which will have a high potential application in the future.

Key words: Myasthenia Gravis, AChR ab, Immunoadsorbent

中图分类号: 

TrendMD: