高等学校化学学报

高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (1): 46.

• 研究简报 • 上一篇    下一篇

镍邻菲绕啉对DNA固定作用的原子力显微镜表征

于力华1, 李壮1, 吴爱国1, 王宏达1, 索全伶2, 毕晓辉3, 黄百渠3   

  1. 1. 中国科学院长春应用化学研究所电分析化学开放研究实验室, 长春 130022;
    2. 内蒙古工业大学化工学院化学系, 呼和浩特, 010064;
    3. 东北师范大学遗传与细胞研究所, 长春 130024
  • 收稿日期:2000-09-11 出版日期:2002-01-24 发布日期:2002-01-24
  • 通讯作者: 李壮(1963年出生),男,研究员,博士生导师,从事扫描探针显微学与生物电化学研究.
  • 基金资助:

    国家自然科学基金(批准号:30070417)资助

The AFM Characterization of Ni(phen)3(2+)Fixing Effects on DNA

YU Li-Hua1, LI Zhuang1, WU Ai-Guo1, WANG Hong-Da1, SUO Quan-Ling2, BI Xiao-Hui3, HUANG Bai-Qu3   

  1. 1. Laboratory of Eletroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
    2. Department of Chemistry, Chemical Engineering Institute, Inner Mongolia Technological University, Hohhot 010064, China;
    3. Institute of Cytology and Genetics, Northeast Normal University, Changchun 130024, China
  • Received:2000-09-11 Online:2002-01-24 Published:2002-01-24

PDF (PC)

被引次数

15

关键词: 原子力显微镜(AFM), 脱氧核糖核酸(DNA), 镍邻菲绕啉, 凝胶电泳

Abstract: In this paper, the fixing and stretching effect of Ni(phen) 2+ 3 with different concentrations on DNAhad been studied by Tapping mode AFM. Under an ambient condition, the high resolution DNAimages were obtained, the average height, width and length of well spread DNAmolecules were measured. The results showed that because of the variations of ionic concentration, the density and topography of DNAmolecules on substrate had a great difference. The AFMand gel electrophoresis results also showed that, under our experimental condition, DNAmolecules kept intact, Ni(phen) 2+ 3 did not catalyze the cleavage activity of EcoRI, therefore, Ni(phen) 2+ 3 would be used to make high resolution physical mapping of DNAby AFM.

Key words: Atomic Force Microscope(AFM), DNA, Ni(phen) 2+ 3, Gel electrophoresis

中图分类号: 

TrendMD: