高等学校化学学报 ›› 2001, Vol. 22 ›› Issue (12): 1996.

• 研究论文 • 上一篇    下一篇

变色酸2C和TAN双显色体系RP-HPLC法分离测定铍(Ⅱ)、铬(Ⅲ)、铜(Ⅱ)、铁(Ⅱ)、镍(Ⅱ)

刘绍璞, 彭敬东, 邓传跃   

  1. 西南师范大学环境化学研究所, 重庆 400715
  • 收稿日期:2000-09-10 出版日期:2001-12-24 发布日期:2001-12-24
  • 通讯作者: 刘绍璞(1941年出生),男,教授,博士生导师,从事分子光谱分析研究.

Separation and Determination of Beryllium(Ⅱ), Chromium(Ⅲ), Copper(Ⅱ), Iron(Ⅱ) and Nickel(Ⅱ) with Two Colour Reagents of Chromotrope 2C and TAN by RP-HPLC

LIU ShaoPu, PENG JingDong, DENG ChuanYue   

  1. Institute of Environmental Chemistry, Southwest Normal University, Chongqing 400715, China
  • Received:2000-09-10 Online:2001-12-24 Published:2001-12-24

摘要: 基于变色酸2C和TAN双显色体系发展了一种同时测定铍(Ⅱ)、铬(Ⅲ)、铜(Ⅱ)、铁(Ⅱ)和镍(Ⅱ)的新RP-HPLC法.在pH=5.0的乙酸盐缓冲介质中,同时加入变色酸单偶氮试剂变色酸2C和噻唑偶氮试剂TAN,它们能与不同金属离子形成有色螯合物,以YWG-ODS柱为固定相,含显色剂、溴化四丁铵和乙酸盐缓冲溶液(pH=5.0)的甲醇/乙醇/水(体积比35:5:60)溶液为流动相,并用分光光度检测器于580nm处测量,用RP-HPLC可成功地分离和测定ng/mL级的铍(Ⅱ)、铬(Ⅲ)、铜(Ⅱ)、铁(Ⅱ)和镍(Ⅱ)等5种金属离子.此方法灵敏度高,简便快速,用于大米、面粉中上述金属离子的测定,结果满意.

关键词: 分离测定金属离子, 变色酸2C, TAN

Abstract: A RP HPLCmethod for the separation and determination of beryllium(Ⅱ), chromium(Ⅲ), copper(Ⅱ), iron(Ⅱ) and nickel(Ⅱ) with chromotrope 2Cand TANchelates on a YWG ODScolumn by using methanol ethanol water(volume ratio is 35:5:60) containing5×10-5 mol/Lchromotrope 2C, 5×10-5 mol/L TANand acetate buffer solution(pH=50) as mobile phase and with spectrophotometric detection at 580 nm was developed. The method has a high sensitivity. The detection limits were 5 μg/mLfor beryllium(Ⅱ), 10 μg/mLfor chromium(Ⅲ), 10 μg/mLfor copper(Ⅱ), 20 μg/mLfor iron(Ⅱ) and 04 μg/mLfor nickel(Ⅱ). Under the optimum conditions most other metal ions do not interfere. The method can be applied to the simultaneous determination of trace amounts of beryllium, chromium, copper, iron and nickel in rice and flour samples.

Key words: Seperation and determination of metal ions, Chromotrope2-C, TAN

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