高等学校化学学报

高等学校化学学报 ›› 2001, Vol. 22 ›› Issue (10): 1664.

• 研究简报 • 上一篇    下一篇

一种金属螯合连续棒色谱柱的制备及色谱性能

卫引茂, 黄晓冬, 耿信笃   

  1. 西北大学现代分离科学研究所, 西安 710069
  • 收稿日期:2000-10-18 出版日期:2001-10-24 发布日期:2001-10-24
  • 通讯作者: 卫引茂(1968年出生),男,博士,副教授,从事生物大分子的分离纯化研究.
  • 基金资助:

    国家自然科学基金(批准号:39880003);陕西省教育委员会专项科学基金(批准号:98JK114)资助

Preparation and Chromatographic Behavior of Continuous Rod Column for Immobilized Metal Affinity Chromatography

WEI Yin-Mao, HUANG Xiao-Dong, GENG Xin-Du   

  1. Institute of Modern Separation Science, Northwest University, Xi'an 710069, China
  • Received:2000-10-18 Online:2001-10-24 Published:2001-10-24

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被引次数

23

关键词: 金属螯合色谱, 连续棒色谱柱, 生物大分子

Abstract: Aprocedure for the preparation of continuous rod consisting of poly(glycidyl methacrylate-co-ethylene dimethacrylate) for immobilized metal affinity chromatography (IMAC) is presented. When chelating Cu(Ⅱ), Zn(Ⅱ) or Ni(Ⅱ) on it, the rod displayed a property of IMACand the selectivity for protein separation was different from that obtained from the naked rod. An abnormal increase in retention times of proteins was found under the condition of either very high pHor very low pHon the metal chelated rods.

Key words: Immobilized metal affinity chromatography, Continuous rod column, Biopolymers

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