高等学校化学学报 ›› 2001, Vol. 22 ›› Issue (10): 1637.

• 研究论文 • 上一篇    下一篇

硅胶及其衍生物作为基体的激光解吸离子化飞行时间质谱

张清春, 邹汉法, 郭忠, 陈小明, 姜泓海, 倪坚毅   

  1. 中国科学院大连化学物理研究所国家色谱研究分析中心
  • 收稿日期:2000-09-12 出版日期:2001-10-24 发布日期:2001-10-24
  • 通讯作者: 邹汉法(1961年出生),男,博士,研究员,博士生导师,主要从事基体辅助激光解吸离子化飞行时间质谱、高效液相色谱及毛细管电色谱的研究.
  • 基金资助:

    国家杰出青年基金(批准号:29725512)资助

Desorption-ionization Mass Spectrometry with Porous Silica Gel and Its Derivatives as Matrix

ZHANG Qing-Chun, ZOU Han-Fa, GUO Zhong, CHEN Xiao-Ming, JIANG Hong-Hai, NI Jian-Yi   

  1. National Chromatographic Reasearch & Analytical Center, Dalian Instiute of Chemical Physics, Chinese Academy of Sciences, Dalian 116011, China
  • Received:2000-09-12 Online:2001-10-24 Published:2001-10-24

摘要: 制备了一种新型无机基体材料(硅胶及其衍生物)用于测定激光解吸离子化飞行时间质谱,建立了一种新的质谱分析方法,并用以解决传统MALDI-TOFMS存在的基体干扰问题.该法适合于低分子量化合物的分析.同时探讨了该新解吸离子化方法的机理.

关键词: 硅胶, 基体, 解吸离子化质谱, 基体固载化, 小分子分析

Abstract: Porous silica gel and its derivatives have been applied as matrix for the analysis of small molecules. In contrast to the conventional MALDI TOF MS, the signal interference of low molecular analytes by the matrix has been eliminated in the MALDI-TOF-M-Swith porous silica gel and its derivatives as the matrix. The detection of limit by this method reached the fetomole levels, and almost no fragment of the analytes has been observed. The porous structure of silica gel is essential for effective desorption and ionization of the analytes. Among several different types of silica gel, the silica gel with the pore size of 30 nm gives the best signal. The analyte penetration into the pore is very important. Matrix agents of DHBand CCAhave been covalently bound to silica gel for the improvement of the intensity of the spectrum signal.

Key words: Silica gel, Matrix, Desorption ionization mass spectrometry, Immobilization of matrix, Small molecule analysis

中图分类号: 

TrendMD: