高等学校化学学报

高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (9): 1364.

• 论文 • 上一篇    下一篇

毛细管区带电泳电化学检测法测定药物及果汁中芦丁和L-抗坏血酸

陈刚, 丁祥欢, 叶建农   

  1. 华东师范大学化学系, 上海 200062
  • 收稿日期:1999-09-18 出版日期:2000-09-24 发布日期:2000-09-24
  • 通讯作者: 叶建农(1947年出生),男,教授,博士生导师,主要从事色谱及电分析化学研究.
  • 基金资助:

    中国科学院长春应用化学研究所电分析化学开放实验室资助

Determination of Rutin and L-Ascorbic Acid in Pharmaceutical Preparations and Fruit Juices by Capillary Zone Electrophoresis with Electrochemical Detection

CHEN Gang, DING Xiang-Huan, YE Jian-Nong   

  1. Department of Chemistry, East China Normal University, Shanghai 200062, China
  • Received:1999-09-18 Online:2000-09-24 Published:2000-09-24

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被引次数

51

摘要： 用毛细管区带电泳-电化学检测法同时测定复方芦丁片及果汁中芦丁和L-抗坏血酸的含量.研究了电极电位、电解液浓度和酸度、电泳电压及进样时间等对电泳的影响,得到了较为优化的测定条件.以直径为300μm的碳圆盘电极为检测电极,电极电位为1.0V(vs.SCE),在25mmol/L硼砂-50mmol/LNaH2PO4(pH8.0)运行缓冲液中,上述两组分在12min内完全分离.芦丁和L-抗坏血酸浓度分别在1.0×10-6～2.5×10-4和5.0×10-6～2.5×10-3mol/L范围内与电泳峰电流呈现良好线性关系,检测下限分别为8.0×10-7和3.3×10-6mol/L.9次测定含5.0×10-5mol/L芦丁和2.5×10-4mol/LL-抗坏血酸的试样溶液,峰高的相对标准偏差分别为2.85%和1.65%,5次测得的平均回收率分别为97.73%和99.68%.

关键词: 毛细管区带电泳, 电化学检测, 芦丁, L

Abstract: A simultaneous detemination of rutin(RT) and L-ascorbic acid(Vc) in pharmaceutical preparations and fruit juices by capillary zone electrophoresis with electrochemical detection(CE-ED) was reported. The effects of potential of working electrode, concentration and pHof the running buffer, separation voltage and injection time on the CE-EDdetermination were investigated and discussed. RTand Vc could be well separated in a 25 mmol/Lborax-50 mmol/L NaH2PO4 buffer(pH8.0) with in 12 min. A 300 μm diameter carbon disc electrode was used as the working electrode positioned carefully opposite the outlet of the capillary in a wall-jet configuration at potential of 1.0 V(vs. SCE). There are excellent linearity between peak current and concentration of analytes in the concentration range of 1.0×10-6—2.5×10-4 mol/L for RT and 5.0×10-6—2.5×10-3 mol/Lfor Vc with the detection limits(S/N=3) of 8.0×10-7 and 3.3×10-6 mol/Lfor RTand Vc, respectively. Relative standard deviations(RSD) of the peak currents of RTand Vc were 2.85%(RT) and 1.65%(Vc) based on 9 determinations of the same sample containing 5.0×10-5 mol/L RTand 2.5×10-4 mol/L Vc. Recoveries of 97.94% for RT and 99.68% for Vc were obtained based on 5 determinations.

Key words: Capillary zone electrophoresis, Electrochemical detection, Rutin, L-Ascorbic acid

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