高等学校化学学报

高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (1): 31.

• 论文 • 上一篇    下一篇

罗红霉素及其代谢物的电喷雾离子阱质谱研究

钟大放, 田蕾, 李雪庆, 许佑君   

  1. 沈阳药科大学药物代谢与药物动力学实验室, 沈阳 110015
  • 收稿日期:1999-03-05 出版日期:2000-01-24 发布日期:2000-01-24
  • 通讯作者: 钟大放(1957年出生),男,教授,主要从事药物质谱分析研究.
  • 基金资助:

    国家自然科学基金(批准号:39625025)资助

Studies on the Electrospray Ion Trap Mass Spectra of Roxithromycin and Its Metabolites

ZHONG Da-Fang, TIAN Lei, LI Xue-Qing, XU You-Jun   

  1. Laboratory of Drug Metabolism and Pharmacokinetics, Shenyang Pharmaceutical University, Shenyang 110015, China
  • Received:1999-03-05 Online:2000-01-24 Published:2000-01-24

PDF (PC)

被引次数

36

摘要： 采用电喷雾离子阱质谱法对人尿样中的罗红霉素及其10种代谢物进行了结构鉴定,利用质谱解析软件对其质谱裂解途径进行分析,发现它们的(+)ESI-MS2和(+)ESI-MS3质谱分别生成脱红霉糖和脱氨基糖碎片,并可见脱去C9位含氮烷基侧链和一系列质荷比相差18的脱水碎片离子,这些特征可用于罗红霉素及其结构类似物的体内代谢转化研究.

关键词: 罗红霉素, 代谢物, 电喷雾离子阱质谱法

Abstract: To investigate the characteristics of mass spectra of roxithromycin and its metabolites, human urine samples which were collected from healthy volunteers after a single dose of 150 mg roxithromycin were analyzed using electrospray ion trap mass spectrometry in a multi-stage MS full scan mode performed on a Finnigan LCQ instrument. The mass spectrometry was operated in the positive mode. The obtained mass spectra were analyzed assisted with the software Mass Frontier 1.0 for their fragmentation passways. Besides roxithromycin, ten of its metabolites were identified in the urine samples by LC/MS. The full scan MS2 and MS3 spectra of each compound gave characteristic fragment ions of descladinose and dedesosamine respectively, and a series of dehydration ions were also found. The difference of fragmentation between the roxithromycin derivatives and the erythromycin oxime derivatives lied in the loss of different fragment ions containing nitrogen. These characteristics can be used for future structure elucidation in the studies of metabolism of roxithromycin and its analogues.

Key words: Roxithromycin, Metabolite, Electrospray ion trap spectrometer

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