高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (4): 634.

• 研究快报 • 上一篇    下一篇

超细Ag颗粒对葡萄糖氧化酶生物传感器响应灵敏度的增强效应

唐芳琼1, 沈继锋1, 张金芳2, 张改莲2   

  1. 1. 中国科学院感光化学研究所, 北京 100101;
    2. 北京师范大学化学系, 北京 100875
  • 收稿日期:1998-11-02 出版日期:1999-04-24 发布日期:1999-04-24
  • 通讯作者: 唐芳琼,女,51岁,副教授.
  • 作者简介:唐芳琼,女,51岁,副教授.
  • 基金资助:

    国家自然科学基金(批准号:69231010,69571032)资助课题

Enhancement of Glucose Biosensor Sensitivity by Addition of Silver Sols

TANG Fang-Qiong1, SHEN Ji-Feng1, ZHANG Jin-Fang2, ZHANG Gai-Lian2   

  1. 1. Institute of Photographic Chemistry, Chinese Academy of Sciences, Beijing, 100101;
    2. Department of Chemistry, Beijing Normal University, Beijing, 100875
  • Received:1998-11-02 Online:1999-04-24 Published:1999-04-24

摘要: 生物传感器在临床医学、环境和食品工业等方面都有重要的用途,且由于其具有体积小、精度高、灵敏度好和便于现场测定等优点,已成为当前研究的热点课题之一.其中研究得较多的是氧化酶电流型生物传感器,特别是介体型,由于其响应快,灵敏而倍受重视.

关键词: 超细Ag颗粒, 葡萄糖氧化酶, 高选择性, 快速反应

Abstract: Anew method for preparation of amperometric glucose oxidase electrode based on enzyme immobilization by Ag sols has been investigated. GOD was simply mixed with Ag nanoparticles and cross linked with a polyvinyl butyl(PVB) medium by glutaradehyde and was coatted on electrode by dipping a platinum wire into the sol-gel solution. In experiments, we discussed the effects of different kinds of silver gels on response of the electrode and the mechanism about it. For the optimum conditions, the current response of the enzyme electrode containing hydrophobic silver gels for 10 mmol/Lglucose was enhanced to 18200 nAas compared with the electrode without silver gels with 280n Acurrent response. In addition to the high sensitivity, the electrode responses rapidly, the 95% steady-state response time is less than 20s.

Key words: Silver nanoparticle, Glucose oxidase, High sensitivity, Rapid response

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