高等学校化学学报

高等学校化学学报 ›› 1998, Vol. 19 ›› Issue (8): 1288.

• 论文 • 上一篇    下一篇

停流法研究Cu(Ser)2及Cu(Gly-Gly)2催化O2·-歧化反应动力学

谢英1, 金虬1, 李大珍1, 高盘良2, 自俊青3   

  1. 1. 北京大学化学系, 北京, 100871;
    2. 大理师范学院化学系, 大理, 671000;
    3. 北京师范大学化学系, 北京, 100875
  • 收稿日期:1997-05-27 出版日期:1998-08-24 发布日期:1998-08-24
  • 通讯作者: 谢英,女,24岁,硕士研究生.
  • 作者简介:谢英,女,24岁,硕士研究生.
  • 基金资助:

    国家自然科学基金(批准号:29775015)资助课题.

The Kinetics Study on Dismutation of Superoxide Catalyzed by Cu(Ser)2and Cu(Gly-Gly)2by Using Stopped-flow Method

XIE Ying1, JIN Qiu1, LI Da-Zhen1, GAO Pan-Liang2, ZI Jun-Qing3   

  1. 1. Deparment of Chemistry, Beijing Normal University, Beijing, 100875;
    2. Dept. of Chem., Peking Univ., Beijing, 100871;
    3. Dept. of Chem., Dali Normal School, Dali, 671000
  • Received:1997-05-27 Online:1998-08-24 Published:1998-08-24

PDF (PC)

摘要： 采用停流法对pH=7.8的磷酸盐缓冲液体系中丝氨酸铜Cu(Ser)2和甘氨酰甘氨酸铜Cu(Gly-Gly)2催化超氧阴离子自由基O2·-歧化反应的动力学进行了研究,求得不同温度下的催化速率常数kcat、反应级数n、活化能Ea及指前因子A,初步探讨了Cu(Ser)2和Cu(Gly-Gly)2催化O2·-歧化反应机理,证实了第一步反应为整体反应过程的速控步骤.

关键词: 超氧阴离子自由基O2·, -, SOD模拟物, 停止流动法, 催化机理

Abstract: In order to estimate the activities of natural SOD and the analogue of SOD on dynamics, the kinetics of Cu(Ser)2and Cu(Gly-Gly)2catalyzed the dismutation of superoxide were studied at pH=7.8 by using stopped-flow method. The rate constants kcat, reaction order n, activation energy Eaand Arrhenius equation were obtained. the reaction mechanism was probed, and it was proved that the first step of the reaction was the determining step.

Key words: Superoxide anion(O2·-), Analogue of SOD, Stopped-flow method, Catalytic mechanism

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