高等学校化学学报 ›› 1998, Vol. 19 ›› Issue (8): 1239.

• 研究简报 • 上一篇    下一篇

血红蛋白在银溶胶上的表面增强喇曼光谱研究

冶保献1, 林琳1, 张悟铭2, 周性尧2   

  1. 1. 郑州大学化学化工学院, 郑州, 450052;
    2. 武汉大学化学系, 武汉, 430072
  • 收稿日期:1997-10-16 出版日期:1998-08-24 发布日期:1998-08-24
  • 通讯作者: 冶保献,男,38岁,博士,副教授.
  • 作者简介:冶保献,男,38岁,博士,副教授.
  • 基金资助:

    国家自然科学基金(批准号:29392604)资助课题.

A Study of Hemoglobin at Silver Colloid by Surface Enhanced Raman Spectroscopy

YE Bao-Xian1, LIN Lin1, ZHANG Wu-Ming2, ZHOU Xing-Yao2   

  1. 1. College of Chemistry and Chemical Engineering, Zhengzhou University, Zhengzhou, 450052;
    2. Department of Chemistry, Wuhan University, Wuhan, 430072
  • Received:1997-10-16 Online:1998-08-24 Published:1998-08-24

摘要: 表面增强喇曼光谱已广泛应用于物质分子在金属表面吸附的研究.人们发现,不仅无机物和有机小分子能产生SERS,而且生物分子,如核酸、色蛋白以及蛋白质均能产生SERS效应,并以此来研究生物分子-蛋白质的变性问题.

关键词: 血红蛋白, 血红素, 表面增强喇曼光谱

Abstract: The properties of Hemoglobin adsorbed on the silver colloid surface were investigated by using surface enhanced Raman spectroscopy(SERS) in the determination of oxygen-hemoglobin, deoxygen-hemoglobin and heme. The results show that the heme chromophore of oxygen-hemoglobin interacts directly with silver colloid, as a result of either its detachment from the protein matrix or denaturation from the protein. The deoxygen-hemoglobin would not release its heme at the silver colloid as normally expected.

Key words: Hemoglobin, Heme, Surface enhanced Raman spectroscopy

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