高等学校化学学报 ›› 1997, Vol. 18 ›› Issue (2): 216.

• 论文 • 上一篇    下一篇

阿达玛变换显微图象分析系统在乳腺肿瘤细胞DNA倍性分析中的应用研究

唐宏武1, 梅二文2, 陈观铨2, 曾云鹗1, 毛永荣3, 何平3, 史宗洁3   

  1. 1. 武汉大学化学系, 武汉, 430072;
    2. 武汉大学分析测试科学系, 武汉, 430072;
    3. 湖北省肿瘤医院, 武汉, 430070
  • 收稿日期:1996-04-09 出版日期:1997-02-24 发布日期:1997-02-24
  • 通讯作者: 唐宏武, 男, 27岁, 博士研究生.
  • 作者简介:唐宏武, 男, 27岁, 博士研究生.
  • 基金资助:

    国家自然科学基金;湖北省自然科学基金

DNA Ploidy Analysis of Breast Tumor Cell by the System of Hadamard Transform Microscopic Image Analysis

TANG Hong-Wu1, MEl Er-Wen2, CHEN Guan-Quan2, ZENG Yun-E1, MAO Yong-Rong3, HE Ping3, SHI Z0ng-Jie3   

  1. 1. Department of Chemistry, Wuhan University, Wuhan, 430072;
    2. Department of Analysis and Measurement Sciencet, Wuhan University, Wuhan, 430072;
    3. Hubei Cancer Hospital, Wuhan, 430070
  • Received:1996-04-09 Online:1997-02-24 Published:1997-02-24

摘要: 以正常人外周静脉血淋巴细胞为标准二倍体细胞,以吖啶橙为细胞DNA荧光探针,用阿达玛变换显微图象分析仪测定了六例乳腺肿瘤的细胞DNA含量(倍性),分析结果与病例学诊断结论吻合,表明该仪器可望用于乳腺癌的诊断和预后研究.研究结果还表明:乳腺肿瘤细胞DNA倍性与核面积(象素)呈显著正相关.

关键词: 阿达玛变换, 图象分析, 乳腺癌, DNA倍性

Abstract: Normal human lymphocyte was used as standard diploid cell, acridine orange was used as fluorescence probe for DNAin cell, and DNAploidy of six cases of human breast tumor was analyzed by the system of Hadamard transform microscopic image analysis.Some parameters were employed to evaluate tumor malignancy, the results are concordant with pathological diagnosis, this indicates that the above instrument may be applied in diagnostic and prognostic study on human breast cancer.The results also show that significant positive correlation exists between tumor cell nuclear DNAploidy and its area (pixels).

Key words: Hadamard transform, Image analysis, Breast cancer, DNA ploidy

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