高等学校化学学报 ›› 1996, Vol. 17 ›› Issue (8): 1198.

• 论文 • 上一篇    下一篇

胶束电动毛细管色谱分析间接紫外吸收检测人体血小板活化因子

何金兰1, 李惠萍2, 陈烈1, 陈伟珍1   

  1. 1. 湛江师范学院化学系, 湛江;
    2. 广东医学院附属医院呼吸病研究室
  • 收稿日期:1995-07-19 出版日期:1996-08-24 发布日期:1996-08-24
  • 通讯作者: 何金兰,女,48岁,副教授.
  • 作者简介:何金兰,女,48岁,副教授.
  • 基金资助:

    广东省高等教育局自然科学基金

Micellar Electrokinetic Capillary Chromatographic Analysis of Platelet Activating Factor in Human Blood by Indirect Ultraviolet Detection

HE Jin-Lan1, LI Hui-ping2, CHEN Lie1, CHEN Wei-Zhen1   

  1. 1. Department of Chemistry, Zhanjiang Teacher's College, Zhanjiang, 524048;
    2. Respiratory Diseases Research Laboratory in Affiliated Hospital of Guangdong Medical Colleg
  • Received:1995-07-19 Online:1996-08-24 Published:1996-08-24

摘要: 研究了人体血小板活化因子中1-O-十六烷基乙酰甘油磷酰胆碱与1-O-八烷基乙酰甘油磷酰胆碱的胶束电动毛细管色谱分离条件与间接紫外吸收检测条件。首次采用高效毛细管电泳法成功地分析了人体血小板活化因子中两种主要磷酰胆碱的含量。

关键词: 胶束电动毛细管色谱, 间接紫外检测, 血小板活化因子

Abstract: O-Hexadecyl-and 1-O-octadecyl-sn-glycero-3-phosphorylcholine(C16-AGEPC and C18-AGEPC) in the platelet activating factor of human blood were analyzed by micellar electrokinetic capillary chromatography (MECC) with indirect ultraviolet absorption detection at 254 nm.The optimum running buffer for the separation was prepared by containing 50 mmol/Lsodium dodecy sulphate-20 mmol/Lpotassium hydrogen phthalate-10 mmol/Lborax-3mol/Lurea(pH=6.8).The separation was completed within 10 min,The detection limits of C16-AGEPC and C18-AGEPC were the same,i.e.60 ng/mL(k=3).The analytical precisions(n=6) were 2.8%~3.0% and 1.4%~1.7% for the determination with peak height and for the measurement of the migration time, respectively,The application of this method to the clinical samples was demonstrated.

Key words: Micellar electrokinetic capillary chromatography, Indirect UV detection, Platelet activating factor

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