高等学校化学学报

高等学校化学学报 ›› 1996, Vol. 17 ›› Issue (6): 883.

• 论文 • 上一篇    下一篇

九种嘌呤碱的毛细管区带电泳分离

赵涛, 刘绮萍, 程介克   

  1. 武汉大学化学系, 武汉 430072
  • 收稿日期:1995-06-03 出版日期:1996-06-24 发布日期:1996-06-24
  • 通讯作者: 程介克
  • 作者简介:赵涛,男,24岁,博士研究生.
  • 基金资助:

    国家自然科学基金

Capillary Zone Electrophoresis Separation of Nine Purine Bases

ZHAO TaO, LIU Qi-Ping, CHENG Jie-Ke   

  1. Department of Chemistry, Wuhan University, Wuhan, 430072
  • Received:1995-06-03 Online:1996-06-24 Published:1996-06-24

PDF (PC)

被引次数

10

摘要： 用毛细管区带电泳法分离和测定咖啡碱、9-N-二羟丙基茶碱、可可碱、腺嘌呤、鸟嘌呤、茶碱、黄嘌呤、尿酸和6-巯基嘌呤。在熔硅毛细管(70cm×75μmi.d.,有效长度63cm)内,使用0.01mol/L(pH=10)硼砂缓冲溶液,电压为25kV,温度为30℃,真空进样1s,于260nm处检测,可在7min内分离上述9种嘌呤碱。理论塔板数达到3.0×105,检测限达fmol级。方法用于人尿分析,结果令人满意。

关键词: 毛细管区带电泳, 嘌呤碱, 人尿

Abstract: This paper reports the separation and determination of caffeine,9-N-dihydroxypropyltheophylline, theobromine, adenine, guanine,theophylline, xanthine, uric acid and 6-mercaptopurine by capillary zone electrophoresis, In an uncoated fused silica capillary(70cm ×75μm i.d., effective length 63 cm), the above mentioned materials can be separated within 7 min in the optimum conditions;0.01 mol/LpH=10 borate buffer,30℃,25 kV,260 nm and 1s load.Efficiency to 3.0×105 the number of theoretical plate is achieved.The detection limit is fmol.The results of urine sample analysis are satisfactory.

Key words: Capillary zone electrophoresis, Purine bases, Urine sample

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