高等学校化学学报

高等学校化学学报 ›› 1996, Vol. 17 ›› Issue (6): 870.

• 论文 • 上一篇    下一篇

甲胎蛋白压电免疫传感器的研究

楚霞, 林朝晖, 沈国励, 俞汝勤   

  1. 湖南大学化学计量学与化学传感技术研究所, 化学化工系, 长沙 410082
  • 收稿日期:1995-06-30 出版日期:1996-06-24 发布日期:1996-06-24
  • 通讯作者: 沈国励
  • 作者简介:楚霞,女,23岁,硕士研究生.
  • 基金资助:

    国家教育委员会博士点基金

Studies on the Piezoelectric Immunosensor for the Determination of α-Fetoprotein (AFP)

CHU Xia, LIN Zhao-Hui, SHEN Guo-Li, YU Ru-Qin   

  1. Institute of Chemometrics and Chemical Sensing Technology, Department of Chemistry and Chemical Engineering, Hunan University, Changsha 410082
  • Received:1995-06-30 Online:1996-06-24 Published:1996-06-24

PDF (PC)

被引次数

34

摘要： 以合成的甲基丙烯酸甲酯和甲基丙烯酸羟乙酯共聚物作载体,在压电石英晶体表面涂覆共聚物涂层,经CNBr活化后固定AFP抗体,研制成AFP压电晶体免疫传感器。研究了固定化方法、载体量、抗原抗体结合时间、重现性、选择性等实验条件和参数的影响。压电晶体的频移值与AFP浓度在100ng/mL～800ng/mL的范围内有良好的线性关系。将此传感器初步用于人体血清样品的测定,并与放射免疫法对照,结果令人满意。

关键词: 压电免疫传感器, 甲胎蛋白, 甲基丙烯酸甲酯-甲基丙烯酸羟乙酯共聚物, 亲和常数

Abstract: Apiezoelectric immunosensor has been developed for the determination of AFP.The crystal is AT-cut and has a basic resonant frequency of 9 MHz.Immobilization of theantibody of AFP(anti-AFP) to the crystal's surface is accomplished via a CNBr-activatedcopolymer coating of hydroxyethyl-methacrylate (HEMA )and methyl-methacrylate(MMA).The effects of immobilization method,the amount of copolymer coating and the reaction time between antigen and antibody are studied.Under the optimized conditions, the frequency shifts are linearly dependent on the AFPconcentrations in the range of 100~800ng/mL.Experimental results show that the piezoimmunosensor has good reproducibility, selectivity and sensitivity.Other antigens presented in human serum do not interfere with the sensor's response.Further, the valent value of AFPand anti-AFPand the affinity constant of immunoreaction in this experimental system are calculated.The analytical results given by this approach are in satisfactory agreement with those given by Radial Immunoassay(RIA).

Key words: Piezoelectric immunosensor, α-Fetoprotein, Copolymer of hydroxyethylmethacrylate and methyl-methacrylate, Affinity constant

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