高等学校化学学报

高等学校化学学报 ›› 1996, Vol. 17 ›› Issue (5): 747.

• 研究简报 • 上一篇    下一篇

经分子设计改造的酵母转录活化因子Ac─[Q236,Q242]GCN4(226—252)—GGC—NH2的化学合成及二聚化

张若衡, 王鹤, 徐筱杰, 唐有棋   

  1. 北京大学物理化学研究所, 北京 100871
  • 收稿日期:1995-03-01 出版日期:1996-05-24 发布日期:1996-05-24
  • 通讯作者: 张若衡,男,36岁,副教授.
  • 作者简介:张若衡,男,36岁,副教授.
  • 基金资助:

    国家“攀登计划”项目

Solid Phase Peptide Synthesis of Designed Yeast Transcriptional Activator Ac [Q236,Q242]GCN4(226─252)─GGC─NH2 and Its Dimerization

ZHANG Ruo-Heng, WANG He, XU Xiao-Jie, TANG You-Qi   

  1. Institute of Physical Chemistry, Peking University, Beijing 100871
  • Received:1995-03-01 Online:1996-05-24 Published:1996-05-24

PDF (PC)

关键词: 酵母转录活化因子, 固相肽合成, 空气氧化

Abstract: Yeast transcriptional activator GCN4(226-252)-GGC-NH2 and the designed Ac-[Q236,Q242)]GCN4(226-252)-GGC-NH2 aiming at altering GCN4 DNA-binding specificity was prepared by stepwise solid phase peptide synthesis.It was found that the yield of the peptide would be greatly improved when treated with dithiothreitol(DTT).The dimer was obtained by air oxidation, which would be used in the sequence-specific DNAbinding activity experiments.

Key words: Yeast transcriptional activator, Solid phase peptide synthesis, Air oxidation

TrendMD: