高等学校化学学报

高等学校化学学报 ›› 1996, Vol. 17 ›› Issue (3): 451.

• 论文 • 上一篇    下一篇

铽离子荧光探针对酶与金属离子间能量转移和结合位的研究

杨景和1, 赵卫东1, 童裳伦1, 揭念琴1, 张桂玲1, 高祖泉2   

  1. 1. 山东大学化学院, 济南 250100;
    2. 山东大学生物系, 济南 250100
  • 收稿日期:1995-03-02 出版日期:1996-03-24 发布日期:1996-03-24
  • 通讯作者: 杨景和,男,54岁,教授.
  • 作者简介:杨景和,男,54岁,教授.
  • 基金资助:

    国家自然科学基金

Terbium(Ⅲ) Luminescence Probes:Determination of Energy Transfer Distance Between Metal Ion and Tryptophan in Proteins and Postulation of Their Binding Sites

YANG Jing-He1, ZHAO Wei-Dong1, TONG Chang-Lun1, JIE Nian-Qin1, ZHANG Gui-Ling1, GAO Zu-Quan2   

  1. 1. College of Chemistry, Shandong University, Jinan 250100;
    2. Department of Biology, Shandong University, Jinan 250100
  • Received:1995-03-02 Online:1996-03-24 Published:1996-03-24

PDF (PC)

被引次数

7

摘要： 应用荧光光度法研究了Tb3+与牛胰盼氧核糖核酸酶(BPD),枯草杆菌α-淀粉酶(BSα-A)的络合发光现象。实验表明,BPD和BSα-A分别在pH=7～8和5～6范围内与Tb3+络合,并发射Tb3+的特征荧光,Tb3+与BPD和BSα-A的络合比分别为2:1和4:1.并应用Forster理论测定了Tb3+与BPD和BSα-A之间能量传递的距离R分别为1.39nm和1.48nm,其临界距离R0分别为0.336nm和0.390nm.推测出Tb3+络合在BPD的178位Trp和BSα-A上的188和321位Trp.

关键词: 铽荧光探针, 能量传递距离, 结合位点

Abstract: The luminescence properties of the complex of Tb(Ⅲ)with bovine pancreatic deoxyribonuclease(BPD),bacillus subtilis α-amylase(BSα-A) were studied by using the fluorescence method.It was found that BPD, BSα-Acan coordinated with Tb(Ⅲ)at pH=7～8 and 5～6 respectively, and then emits a strong characteristic fluorescence of Tb(Ⅲ).The complex ratios for Tb (Ⅲ) and BPD, BSα-Awere 2:1 and 4:1 respectively.In this paper,Forster theory was used to determine the energy transfer distance between BPD,BSα-Aenergy-donor and Tb (Ⅲ) energy-acceptor.The values of the critical distance for 50% energy transfer,R0,of 0.336 nm and 0.390 nm were obtained.The donor-acceptor energy transfer distances,R,estimated from the measured efficiency were 1.39 nm,1.48 nm respectively.It is inferred that the binding sites between Tb (Ⅲ) and BPD, BSα-Awere Trp-178, Trp-188 and Trp-321,respectively.

Key words: Terbium luminescence, Energy transfer distance, Binding sites

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