高等学校化学学报

高等学校化学学报 ›› 1996, Vol. 17 ›› Issue (3): 424.

• 论文 • 上一篇    下一篇

曼氏血吸虫合成多肽抗原研究

许家喜1, 蔡孟深2, 石佑恩3   

  1. 1. 北京大学化学与分子工程学院, 北京 100871;
    2. 北京医科大学药学院;
    3. 同济医科大学寄生虫系
  • 收稿日期:1995-04-17 出版日期:1996-03-24 发布日期:1996-03-24
  • 通讯作者: 许家喜,男,32岁,博士,副教授.
  • 作者简介:许家喜,男,32岁,博士,副教授.
  • 基金资助:

    国家博士后科研流动站基金

Studies on the Synthetic Peptied Antigens of Schistosoma Mansoni

XU Jia-Xi1, CAI Meng-Shen2, SHI You-En3   

  1. 1. School of Chemistry and Molecular Engineering, Peking University, Beijing 100871;
    2. School of Phamarceutical Sciences, Beijing, Medical University;
    3. Department of Parasitology, Tongji Medical University
  • Received:1995-04-17 Online:1996-03-24 Published:1996-03-24

PDF (PC)

被引次数

9

摘要： 在计算机的辅助下,根据曼氏血吸虫26KDa谷胱甘肽S-转移酶的氨基酸序列,通过对其亲水性、柔韧性、可接近性、电荷分布和二级结构的分析来预测出6个抗原肽,并用固相法进行了合成。经Dot-ELISA法测定,其中的2个对抗日本血吸虫免疫球蛋白多克隆抗体(抗-SJ-IgGPcAb),1个对抗血吸虫表膜单克隆抗体(A6McAb)均显示良好的抗原性。

关键词: 曼氏血吸虫, 谷胱甘肽S-转移酶, 抗原肽, 多肽疫苗

Abstract: Six antigenic peptides of the 26KDa Glutathione S-transferase of schistosoma mansoni have been predicted according to its hydrophilicity, flexibility,accessibility,chargedistribution and β-turn in the secondary structure by the determination of its primary structure with the aid of computer and synthesized by using solid phase method.Two of them showed a good antigenicity by Dot-ELISAmethod with anti-schistosoma japonicum-immunoglobulin polyclonal antihody,anti-Sj-IgG PcAb and one of them also showed a good antigenicity with the anti-schistosoma surface membrane monoclonal antibody,A6McAb.

Key words: Schistosoma mansoni, Glutathione S-transferase, Antigenic peptide, Peptide vaccine

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