高等学校化学学报 ›› 1996, Vol. 17 ›› Issue (11): 1702.

• 论文 • 上一篇    下一篇

筛选免疫药物的单扫描示波极谱法研究

亢晓峰1, 宋俊峰2, 胡荫华2   

  1. 1. 宝鸡文理学院化学化工系, 宝鸡, 721007;
    2. 西北大学电分析化学研究所, 西安, 710069
  • 收稿日期:1995-12-26 出版日期:1996-11-24 发布日期:1996-11-24
  • 通讯作者: 亢晓峰,男,32岁,讲师.
  • 作者简介:亢晓峰,男,32岁,讲师.
  • 基金资助:

    国家自然科学基金;陕西省高校专项科学基金

Studies on Screening Immune Drugs by Linear Sweep Polarography

KANG Xiao-Feng1, SONG Jun-Feng2, HU Yin-Hua2   

  1. 1. Department of Chemistry and Chemical Engineering, Baoji College of Arts and Science, Baoji, 721007;
    2. Institute of Electroanalytical Chemistry, Northwest University, Xian, 710069
  • Received:1995-12-26 Online:1996-11-24 Published:1996-11-24

摘要: 用示波极谱研究了溶血素的溶血活性,在1/15mol/LNa2HPO4-1/15mol/LKH2PO4(pH=7.4)底液中活性溶血素有一灵敏的极谱阴极波,峰电位为-1.82V(vs.SCE),峰高与溶血素活性在6.0×10-6~5.3×10-5unit/mL和5.3×10-5~2.0×10-3unit/mL范围内呈线性关系,根据不同药物对抗血清中溶血素形成的不同影响,建立了极谱法筛选免疫药物的新方法。本法简单、快速,并且溶血素的测定不再依赖于免疫溶血过程,结果比较准确、客观。

关键词: 溶血素, 极谱法, 免疫药物

Abstract: The hemolytic activity of hemolysin was investigated by linear sweep polarography in this paper.Asensitive polarographic reduction wave of hemolysin was observed in 1/15 mol/L Na2HPO4~1/15 mol/L KH2PO4 buffer solution(pH=7.4).The peak potential was -1.82 V(vs.SCE).The peak height was linearly proportional to its biological activity in the ranges of 6.0×10-6~5.3×10-5 unit/mL and 5.3×10-5~2.0×10-3 unit/mL.According to the various effects of drugs on the formation of hemolysin antibody,a new method for screening immune drugs was established.This method is simple,rapid,and the assay of hemolysin activity is no longer dependent upon immune hemolysis process.The results are accurate and reliable.

Key words: Hemolysin, Polarography, Immune drug

TrendMD: