高等学校化学学报 ›› 1995, Vol. 16 ›› Issue (9): 1377.

• 研究简报 • 上一篇    下一篇

电导法测脲酶活性

朱元保, 颜流水, 何双娥   

  1. 湖南大学化学化工系, 长沙, 410082
  • 收稿日期:1994-09-29 修回日期:1995-05-29 出版日期:1995-09-24 发布日期:1995-09-24
  • 通讯作者: 朱元保,男,56岁,教授.
  • 作者简介:朱元保,男,56岁,教授.
  • 基金资助:

    国家自然科学基金

Determination of Urease Activity by Conductometry

ZHU Yuan-Bao, YAN Liu-Shui, HE Shuang-E   

  1. Department of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082
  • Received:1994-09-29 Revised:1995-05-29 Online:1995-09-24 Published:1995-09-24

关键词: 脲酶活性, 电导法, 米氏常数, 活化能

Abstract: A rapid and simple conductometric method has been estabilished for the assay of urease activity.Experimental results showed that the method has the advantages of high sensitivity and wide linear range of 0. 02 to 0. 03U/ mL,and does not need other reagents except substrate.The Michaelis constant and activation energy of the enzymatical reaction were also determined,which are in good agreement with previously reported values.Furthermore,the proposed method had been applied to assay urease content from swordbean and soybean with a RSD within 0.8% and a recovery of 95% to 105%.

Key words: Urease activity, Conductometry, Michaelis constant, Activation energy

TrendMD: