高等学校化学学报

高等学校化学学报 ›› 1995, Vol. 16 ›› Issue (8): 1212.

• 研究简报 • 上一篇    下一篇

NAD-硅胶亲和色谱固定相的合成和在核苷酸及其碱基分析中的应用

于世林, 苗凤琴, 杨朝霞, 冯茹   

  1. 北京化工大学应用化学系, 北京, 100029
  • 收稿日期:1994-08-18 修回日期:1995-01-24 出版日期:1995-08-24 发布日期:1995-08-24
  • 通讯作者: 于世林,男,56岁,教授.
  • 作者简介:于世林,男,56岁,教授.
  • 基金资助:

    国家自然科学基金

The Application of an NAD-Silica Biospecific Affinity Chromatography Stationary Phase in Analysis of Nucleotides

YU Shi-Lin, MIAO Feng-Qin, YANG Zhao-Xia, FENG Ru   

  1. Dept.of Appl. Chem., Beijing University of Chemical Technology, 100029
  • Received:1994-08-18 Revised:1995-01-24 Online:1995-08-24 Published:1995-08-24

PDF (PC)

被引次数

1

关键词: 烟酰胺腺嘌呤二核苷酸(NAD), 亲和色谱, 核苷酸

Abstract: By using a wide-pore(30 nm),microspheric silica(8μm)as the matrix and γaminopropyltriethoxysilane as the coupling agent,a stationary phase of biospecific affinity chromatography was prepared by chemical binding nicotinamide adenine dinucleotide(NAD) to the γ-aminopropyl modified silica. Using the phosphate buffer(pH=7.0)as a mobile phase,the separation of some nucleotides is accomplished by the detection of UVD(260 nm).The effect of changed ion strengh of eluent on the resolution of nucleotide(AMP, ADP and ATP)has been studied.

Key words: NAD, Affinity chromatography, Nucleotide

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